Evaluation of amitriptyline-induced toxicity in freshly isolated rat hepatocytes and the protective role of taurine
- سال انتشار: 1392
- محل انتشار: همایش ملی پژوهش های کاربردی در علوم و مهندسی
- کد COI اختصاصی: TIAU01_825
- زبان مقاله: انگلیسی
- تعداد مشاهده: 978
نویسندگان
Students’ Research Committee, Faculty of pharmacy, Tabriz University of medical science, Tabriz, Iran
Drug applied research center, Tabriz University of medical science, Tabriz, Iran.
Students’ Research Committee, Faculty of pharmacy, Tabriz University of medical science, Tabriz, Iran Drug applied research center, Tabriz University of medical science, Tabriz, Iran.
چکیده
Administration of amitriptyline, a most commonly used tricyclic antidepressant, is associated with anticholinergic side effects and rare but severe hepatotoxicity. It supposed that the intermediated metabolites of amitriptyline produced by CYP450 involved in hepatotoxicity but the exact mechanisms involved in hepatotoxicity are unknown.The aim of this study was to determine the mechanism of hepatotoxicity induced by amitriptyline and protectiverole of taurine in an in vitro model of isolated rat hepatocytes. Markers such as cell viability, reactive oxygen species (ROS) formation, lipid peroxidation, mitochondrial membrane potential, and hepatocytes glutathione content were evaluated every 60 minutes for 180 minutes. Our results showed that amitriptyline resulted incytotoxicity characterized by the reduction in cell viability, an increase in ROS formation and lipid peroxidation, mitochondrial membrane potential collapse, and a reduction in cellular glutathione content. Our finding showedadministration of taurine (1mM) effectively reduced the toxic effects of amitriptyline in isolated rat hepatocytesکلیدواژه ها
Amitriptyline, Taurine, Oxidative stress, Hepatocytesاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.