F۲ peptide fraction of Androctonus crassicauda scorpion venom: Inducing M۲ to M۱ macrophage polarization and inhibiting colon carcinoma cell proliferation and migration
- سال انتشار: 1404
- محل انتشار: مجله گیاهان دارویی ابن سینا، دوره: 15، شماره: 5
- کد COI اختصاصی: JR_AJP-15-5_011
- زبان مقاله: انگلیسی
- تعداد مشاهده: 42
نویسندگان
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Venomous Animals and Anti-venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Objective: Colorectal cancer (CRC) is among the deadliest malignancies, often diagnosed at advanced stages, limiting treatment efficacy and necessitating alternative therapeutic approaches. Scorpion venom has emerged as a promising source of bioactive compounds for cancer therapy. This study investigated the anti-cancer potential of Androctonus crassicauda scorpion venom fractions against CT-۲۶ colon cancer cells.Materials and Methods: A. crassicauda venom fractions were isolated using gel filtration chromatography. Murine peritoneal macrophages, harvested from BALB/c mice, were polarized towards the M۲ phenotype and characterized by flow cytometry. Real-time PCR and ELISA quantified M۱ and M۲ macrophage-associated gene and cytokine expression. The impact of venom fractions on CT-۲۶ cell proliferation and migration was assessed via MTT and wound-healing assays. Phagocytic activity was evaluated using a yeast phagocytosis assay.Results: The F۲ venom fraction significantly upregulated pro-inflammatory gene and cytokine expression, and downregulated anti-inflammatory gene and cytokine expression in M۲ macrophages. Furthermore, the F۲ fraction significantly inhibited CT-۲۶ cell proliferation and migration. Critically, it also enhanced the phagocytic capacity of M۲ macrophages.Conclusion: Our results suggest that the F۲ fraction of A. crassicauda scorpion venom reprograms tumor-associated M۲ macrophages towards an anti-tumor M۱ phenotype. These findings suggest the potential of the F۲ fraction of A. crassicauda scorpion venom as a novel therapeutic strategy for the treatment of colon cancer. However, to confirm this potential, further in vivo studies need to be carried out.کلیدواژه ها
Colorectal cancer, Scorpion venom, Androctonus crassicauda Cancer therapy, Macrophage polarizationاطلاعات بیشتر در مورد COI
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