Exploration of Active Ingredients and Anti-Inflammatory Mechanisms of Caulerpa Lentillifera Extracted by Enzyme Method
- سال انتشار: 1404
- محل انتشار: Iranian Journal of Chemistry and Chemical Engineering، دوره: 44، شماره: 7
- کد COI اختصاصی: JR_IJCCE-44-7_013
- زبان مقاله: انگلیسی
- تعداد مشاهده: 27
نویسندگان
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, P.R. CHINA
School of Marine Biology and Fisheries, Hainan University, P.R. CHINA
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, P.R. CHINA
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, P.R. CHINA
Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, P.R. CHINA
Hainan Academy of Ocean and Fisheries Sciences, P.R. CHINA
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, P.R. CHINA
Hainan Academy of Ocean and Fisheries Sciences, P.R. CHINA
چکیده
This article deeply studied the active ingredients in Caulerpa lentillifera (known as “sea grapes”) through enzymatic extraction technology, and explored its anti-inflammatory mechanism. In the study, the extraction conditions were optimized, including enzyme type, concentration, reaction temperature, time, and pH value, successfully improving the extraction efficiency. Tests have shown that under the conditions of ۰.۵% w/w cellulase and pectinase, at a reaction temperature of ۵۰ °C and an extraction time of ۳ hours, with a pH value of ۵, the content of polyphenols and flavonoids in Caulerpa lentillifera is the highest, reaching ۲۲ mg/g and ۱۲.۲ mg/g, respectively. Through in vitro cell culture experiments, it was found that Caulerpa lentillifera extract can effectively reduce the levels of inflammatory factors IL-۶ and TNF-α. Among them, IL-۶ and TNF-α levels treated with ۱۰۰μg/mL extract were reduced to ۶۵±۸ pg/mL and ۷۵±۹ pg/mL, respectively, demonstrating good anti-inflammatory effects. In vivo experiments have also confirmed the anti-inflammatory effect of Caulerpa lentillifera extract, significantly reducing the levels of inflammatory factors in LPS (Lipopolysaccharide) induced acute inflammation mouse models and improving liver and kidney function indicators. Molecular mechanism experiments have shown that Caulerpa lentillifera extract inhibits inflammatory responses by downregulating the activity of inflammatory factors and the NF-KB (Nuclear Factor kappa B) signaling pathway. These data not only demonstrate the efficient extraction method of active ingredients from Caulerpa lentillifera but also reveal their potential application value in anti-inflammatory therapy.کلیدواژه ها
Enzyme Extraction, Caulerpa Lentillifera, Active ingredients, Mechanisms of Anti-inflammatory Action, nuclear factor kappa Bاطلاعات بیشتر در مورد COI
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