Design and Fabrication of a New Image Processing-Based Sensor for Continuous Non-Invasive Monitoring of Cell Density
- سال انتشار: 1404
- محل انتشار: Iranian Journal of Chemistry and Chemical Engineering، دوره: 44، شماره: 7
- کد COI اختصاصی: JR_IJCCE-44-7_014
- زبان مقاله: انگلیسی
- تعداد مشاهده: 13
نویسندگان
Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, I.R. IRAN
Department of Biology, Faculty of Basic Science, Ale Taha Institute of Higher Education, Tehran, I.R. IRAN
Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, I.R. IRAN
Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, I.R. IRAN
چکیده
A new image-processing-based turbidity sensor has been designed, built, and evaluated for non-invasive, real-time cell growth monitoring. The sensor employs advanced turbidimetric measurement methods without traditional optical sensors and achieves greater measurement precision. The major steps in its development included sensor design, optimization of light transmittance, image processing software development, and sensor calibration. The sensor was validated using real-time monitoring of recombinant E. coli in High-Cell-Density Culture (HCDC) within a bioreactor. Calibration involved correcting light intensity for accurate culture monitoring and cross-referencing with offline biomass measurements. Capable of measuring up to ۵۲ g/L (OD۶۲۰=۱۱۶) of cell concentration without any sample pretreatment, the system is ideally suited for industrial bioreactors. This technology enhances bioprocess automation by providing accurate, real-time biomass measurements without dilution, offering a low-cost and scalable industrial solution.کلیدواژه ها
Turbidity Sensor, Real-time monitoring, Optical Density, Biomass measurement, High cell-density cultivationاطلاعات بیشتر در مورد COI
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