Assessing the Reducing Effect of Coenzyme Q۱۰ on Carbendazim-Induced Testicular Tissue Dysfunction Through Modulation of miR-۲۰۲-۵p/Apoptosis Signaling in Rats: A Histological and Immunohistochemical Study
- سال انتشار: 1404
- محل انتشار: دوفصلنامه علوم و فنون دامپزشکی ایران، دوره: 17، شماره: 2
- کد COI اختصاصی: JR_IJVST-17-2_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 55
نویسندگان
Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran.
Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran.
Department of Anatomy and Histology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq.
چکیده
Widespread application of carbendazim (Carb) in agriculture and veterinary is a major environmental concern because of its residues that disrupt spermatogenesis. Recently, coenzyme Q۱۰ (CoQ۱۰) supplementation has demonstrated various health benefits due to its anti-apoptosis and anti-inflammatory nature. Thus, the present study aimed to investigate the possible mechanistic pathway of CoQ۱۰ in Carb-induced reproductive dysfunction in male rats. Adult male Wistar rats were orally exposed to Carb (۱۵۰ mg/kg) singly or in combination with CoQ۱۰ (۲۰۰ mg/kg). The rats received their treatments daily for ۹ weeks. At the end of the work, the testis specimens were excised for histological (H & E staining), immunohistochemical, hormonal, and molecular (real-time quantitative PCR) assessments. The Carb group showed adverse testicular alterations confirmed by immunostaining and demonstrated a significant upregulation of Bax and Caspase-۳ expression, while exhibiting a notable reduction in the immunopositivity of Bcl-۲ protein within the testes of rats. Real-time PCR analysis revealed that Carb treatment decreased the expression of miR-۲۰۲-۵p with a concomitant decline in concentrations of testosterone and LH hormones. Conversely, in Carb-treated rats, co-treatment with CoQ۱۰ restored the tissue architecture, hormonal disturbance, and declined apoptotic index to near control level. In addition, high expression of miR-۲۰۲-۵p was observed in the Carb + CoQ۱۰ group, and testicular tissues returned to nearly normal histological architecture. We concluded that Carb causes adverse testicular alterations via miR-۲۰۲-۵p/apoptosis pathway, and CoQ۱۰ may prove useful in combating Carb-induced adverse effects via its anti-apoptotic and gene regulatory effects.کلیدواژه ها
histology, Testis, Carbendazim, Rat, Coenzyme Q۱۰اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.