Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces
- سال انتشار: 1404
- محل انتشار: دوفصلنامه علوم و فنون دامپزشکی ایران، دوره: 17، شماره: 1
- کد COI اختصاصی: JR_IJVST-17-1_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 40
نویسندگان
Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
چکیده
Actinomycetes are a vital group of Gram-positive bacteria known for producing a wide range of bioactive secondary metabolites, including important antibiotics. These microorganisms play an essential role in the degradation of organic matter and nutrient cycling, contributing significantly to soil health and fertility. Their capacity to synthesize diverse compounds and the presence of key biosynthetic pathways involving polyketide synthases and non-ribosomal peptide synthetases highlights their potential in antibiotic discovery, particularly against antibiotic-resistant pathogens. This study aimed to isolate and characterize Actinomycetes from fresh sheep feces collected in Ilam Province, Iran, focusing on their antibacterial activity and biosynthetic potential. A total of ۸۶ actinomycete isolates were obtained from fecal samples collected from sheep in ۲۰۲۱. Morphological characterization confirmed all isolates as Gram-positive and filamentous. Molecular identification through PCR amplification of the ۱۶S rRNA gene yielded a product of approximately ۶۴۰ base pairs for all isolates. Antibacterial screening revealed that ۱۷ isolates exhibited activity against various pathogens, with the highest efficacy observed against Bacillus cereus (۶۲.۱%). Molecular analysis also indicated the presence of biosynthetic gene clusters, with ۳۱ isolates (۳۶.۰۵%) bearing non-ribosomal peptide synthetase (NRPS) gene, ۱۵ isolates (۱۷.۴۴%) containing polyketide synthase I (PKS-I), and ۱۶ isolates (۱۸.۶%) with polyketide synthase II (PKS-II) genes. This study highlights the significant antibacterial properties and biosynthetic capabilities of actinomycetes from sheep feces, suggesting their potential use in therapeutic, agriculture, and biotechnological applications.کلیدواژه ها
Actinomycetes, Polyketide Synthase, Non-Ribosomal Peptide Synthetase, Antibacterial activity, Sheep Fecesاطلاعات بیشتر در مورد COI
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