A High-Throughput and Green Planar Chromatographic Approach for Furosemide Analysis in Plasma
- سال انتشار: 1404
- محل انتشار: نشریه آسیایی شیمی سبز، دوره: 9، شماره: 5
- کد COI اختصاصی: JR_AJGC-9-5_009
- زبان مقاله: انگلیسی
- تعداد مشاهده: 36
نویسندگان
Bharati Vidyapeeth’s College of Pharmacy, Sector ۸, CBD Belapur, Navi Mumbai, Maharashtra – ۴۰۰۶۱۴, India
Bharati Vidyapeeth’s College of Pharmacy, Sector ۸, CBD Belapur, Navi Mumbai, Maharashtra – ۴۰۰۶۱۴, India
Bharati Vidyapeeth’s College of Pharmacy, Sector ۸, CBD Belapur, Navi Mumbai, Maharashtra – ۴۰۰۶۱۴, India
Bharati Vidyapeeth’s College of Pharmacy, Sector ۸, CBD Belapur, Navi Mumbai, Maharashtra – ۴۰۰۶۱۴, India
Amity Institute of Biotechnology, Amity University, Mumbai - Pune Expressway, Bhatan, Mumbai, Maharashtra – ۴۱۰۲۰۶, India
Bharati Vidyapeeth’s College of Pharmacy, Sector ۸, CBD Belapur, Navi Mumbai, Maharashtra – ۴۰۰۶۱۴, India
چکیده
A high-performance thin-layer chromatography (HPTLC) method was developed and validated to quantify furosemide in human plasma, providing a rapid, cost-effective, and environmentally sustainable alternative to conventional chromatographic techniques. The method employed silica gel ۶۰ F۲۵۴ precoated TLC plates with a mobile phase consisting of isopropyl alcohol, formic acid, ethyl acetate, chloroform, and toluene (۱.۵:۱:۰.۵:۳:۴, v/v/v/v/v), and densitometric detection at ۲۷۱ nm. Paracetamol was used as an internal standard. The method exhibited a strong linear correlation over the ۳۰۰-۲۱۰۰ ng/band concentration range with a correlation coefficient (r²) of ۰.۹۹۴۹. The lower limit of quantification was ۵۰ ng/band, demonstrating high sensitivity. The intra-day and inter-day precision (%CV) were less than ۲%, and the accuracy ranged from ۸۵.۴۹% to ۹۴.۴۶%. Recovery was consistent across all QC levels, with a maximum of ۸۴.۷۰%, and dilution integrity yielded ۱۱۱% recovery. The method proved robust under slight variations in chromatographic conditions and showed acceptable stability under benchtop, freeze-thaw, overnight, and long-term storage conditions. Overall, this HPTLC method is simple, reproducible, and suitable for high-throughput applications, such as pharmacokinetic studies, bioequivalence assessments, and therapeutic drug monitoring of furosemide in human plasma.کلیدواژه ها
Planar Chromatography, Bioanalytical Method Validation, Green Analytical Chemistry, High-Performance Thin-Layer Chromatography (HPTLC), Analytical Method Developmentاطلاعات بیشتر در مورد COI
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