Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG۲ Cell Line
- سال انتشار: 1399
- محل انتشار: Iranian Journal of Chemistry and Chemical Engineering، دوره: 39، شماره: 5
- کد COI اختصاصی: JR_IJCCE-39-5_013
- زبان مقاله: انگلیسی
- تعداد مشاهده: 61
نویسندگان
Department De Bioquimica I De Biologia Molecular, Institut De Biotecnologia Biomedicina (BB), Universitat Autonoma De Barcelona, Barcelona, SPAIN
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, I.R. IRAN
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, I.R. IRAN
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University Tehran, I.R. IRAN
چکیده
Noscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants in the Papaveraceae family which this benzylisoquinoline alkaloid and its synthetic subsidiaries (called noscapinoids) are being assessed for their anticancer potential. The present research aimed to investigate the induction of DNA destruction and viability of HepG۲ tumor spheroid culture influenced by noscapine and nanosuspension of noscapine. Culture of HepG۲ cells as spheroids was treated with different concentrations of noscapine for ۲۴ h on Day ۱۱. Afterward, viability assay and alkaline comet assay methods were applied to examine the viability and induced DNA destruction, respectively. Based on the results, no significant impact was observed from Tween ۴۰ on the viability and DNA damage levels in comparison with the control (p > ۰.۰۵). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability of hepatic cancer cells and elevation of DNA damages, showing a correlation between rises of DNA damages and viability decline.کلیدواژه ها
Genotoxicity, Noscapine, Nanosuspension, Microfluidic Reactors, HepG۲اطلاعات بیشتر در مورد COI
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