N-(۴-(۴-Chlorophenyl)Thiazol-۲-yl)-۲-(۲-Phenylacetamido) Acetamide Derivatives as Potential Anticancer Agents: Synthesis and Cytotoxicity Evaluation
- سال انتشار: 1403
- محل انتشار: Iranian Journal of Chemistry and Chemical Engineering، دوره: 43، شماره: 6
- کد COI اختصاصی: JR_IJCCE-43-6_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 66
نویسندگان
Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, I.R. IRAN
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, I.R. IRAN
Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, I.R. IRAN
Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, I.R. IRAN
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, I.R. IRAN
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, I.R. IRAN
چکیده
According to the rising statistics of cancer cases, the discovery of novel anticancer drugs is a critical issue in current medical research. Besides, drug resistance and the incidence of severe adverse effects are the logical reasons for the discovery of new antineoplastic agents. According to the positive background that has been observed for ۱,۳-thiazole derivatives as potential anticancer drugs, we decided to synthesize a new series of ۱,۳-thiazole-based cytotoxic agents. MTT assay, activation of caspase ۳, capability for reduction of the Mitochondrial Membrane Potential (MMP), and production potency of Reactive Oxygen Species (ROS) were investigated. Some of the tested compounds demonstrated potent cytotoxic activity and also caspase ۳ activation, MMP reduction, and ROS generation. Three cancerous cell lines namely Hela (cervical cancer), A۵۴۹ (lung carcinoma), and U۸۷ (glioblastoma) were applied to perform the MTT assay. Diverse moieties with different electronic features were substituted on the phenyl ring to reveal the structure-activity relationships of the target compounds ۸a-۸o. Hela (cervical cancer), A۵۴۹ (lung carcinoma), and U۸۷ (glioblastoma) were utilized as cancerous cells to explore the cytotoxicity via MTT Hela and U۸۷ cells were more sensitive to the tested compounds and A۵۴۹ was more resistant to the tested derivatives. Compound ۸a with ortho chlorine moiety on the phenyl ring was the most active derivative against Hela cells (IC۵۰ = ۱.۳±۰.۱۴ µM). All evaluated derivatives rendered lower activity against A۵۴۹ than doxorubicin as a standard anticancer drug. Only some of the evaluated compounds showed more cytotoxicity against U۸۷ than doxorubicin. Furthermore, caspase ۳ activation, Mitochondrial Membrane Potential (MMP), and Reactive Oxygen Species (ROS) generation were also investigated. Fortunately, some of the tested compounds were also active in these tests. In conclusion, the current ۱,۳-thiazole derivatives could be suggested as potential anticancer lead compounds.کلیدواژه ها
Synthesis, ۱, ۳-Thiazole, Phenylacetamide, Cytotoxicity, Caspase ۳اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
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