Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle-Bed Bioreactor-Involvement of Ligninolytic Enzymes
- سال انتشار: 1388
- محل انتشار: Iranian Journal of Chemistry and Chemical Engineering، دوره: 28، شماره: 2
- کد COI اختصاصی: JR_IJCCE-28-2_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 10
نویسندگان
Department of Applied Chemistry and Chemical Engineering, University of Tabriz, Tabriz, I.R. IRAN
Department of Chemical Engineering, Amirkabir University of Technology, Tehran, I.R. IRAN
Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC CANADA
Department of Chemical Engineering, Amirkabir University of Technology, Tehran, I.R. IRAN
چکیده
The decolorization of Maxilon-red dye by Kissiris immobilized Phanerochaete chrysosporium in the trickle-bed reactor (TBR) using the basal nitrogen-limited growth medium was studied. The influence of the superficial liquid velocity (SLV) on the decolorizing ability of the fungus was examined at four SLVs (cm sec-۱): ۰.۰۵, ۰.۰۷۵, ۰.۱, and ۰.۱۵. Maximum level of the decolorizing activity was about ۹۴ % by day ۴-۵ of the process when the TBR operated at SLV=۰.۰۷۵ to ۰.۱ cm sec-۱. At these SLVs, the maximum activities of the lignin peroxidase (LiP) and the manganese peroxidase (MnP) were (UL-۱): ۸۷ and ۲۰۷, respectively. In a successive decolorization of the tested dye in a continuous dye addition in the TBR when glucose level decreased to ۲ gL-۱, significant reduction in the characteristics of the interest were observed. While by controlling the glucose concentration, these properties were improved considerably: The decolorizing ability of the Kissiris immobilized mycelia was more than ۸۵ % after nearly ۲۰ days of the process while the maximum activities of the LiP and the MnP were (UL-۱): ۳۲۰ and ۱۵۱, respectively. Considerable levels of the enzyme activities were detected by day ۱۹ and after ۱۱ times of the dye additions.کلیدواژه ها
Ligninolytic enzymes, Kissiris-immobilized Phanerochaete chrysosporium, Decolorization, Maxilon-red synthetic dye, Trickle-bed bioreactorاطلاعات بیشتر در مورد COI
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