Preparation and Evaluation of Curcumin Nano Emulsion to Inhibit TC-۱ Cell Growth
- سال انتشار: 1403
- محل انتشار: Iranian Journal of Chemistry and Chemical Engineering، دوره: 43، شماره: 11
- کد COI اختصاصی: JR_IJCCE-43-11_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 64
نویسندگان
Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, I.R. IRAN
Active Pharmaceutical Ingredients Research Center (APIRC), Tehran Medical Sciences, Islamic Azad University, Tehran, I.R. IRAN
Herbal Pharmacology Research Center, School of Medicine, Department of Pharmacology, Tehran Medical Sciences, Islamic Azad University, Tehran, I.R. IRAN
Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, I.R. IRAN
Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, I.R. IRAN
چکیده
Curcumin (Cur), a substance originating from Curcuma longa, has been comprehensively examined for its anticancer properties. Nonetheless, its clinical application is restricted by its inadequate solubility, bioavailability, and stability. TC-۱ cells have been impressive in understanding HPV biology and developing therapeutic approaches for HPV infection and related cancers, like cervical cancer, offering a close mimicry of HPV-induced carcinogenesis. This study's primary goal is to formulate and optimize curcumin nanoemulsion (Cur-NE) to address these challenges and, secondarily, evaluate their impact on TC-۱ cell growth. The characterization of the nanoemulsions was performed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and High-performance liquid chromatography (HPLC). The results revealed an average particle size of ۵۲.۵ nm, a zeta potential of -۱۳.۱ mV, and a drug content of ۹۶.۳%. The dialysis diffusion technique demonstrated that the release of Cur from Cur-NE is slower and more sustained than free Cur. According to an MTT assay, Cur-Ne with an IC۵۰ ۳۵ μg/mL exhibited an increased inhibitory effect of Cur on TC-۱ cancer cells while showing no inhibitory effects on MC۳T۳ normal cells at concentrations up to ۱۰۰ μg/mL. In summary, this study highlights the potential of nanoemulsions as effective carriers for Cur, and their safety is demonstrated in both cancer and normal cells. In addition, Cur-NE showed significant inhibitory activity against TC-۱ cancer cells, suggesting its promising ability in treating HPV-related cancers, especially cervical cancer. Further studies are warranted to evaluate the long-term safety of this nanoemulsion for clinical trials and its efficacy against other cancer cell lines.کلیدواژه ها
curcumin, Cytotoxicity, Nanoemulsion, TC-۱ cell, HPVاطلاعات بیشتر در مورد COI
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