Hyaluronic Acid/Chitosan Hydrogel: Biomimetic Wound Dressing Inspired by the Inherent Structure of the Skin on Diabetic Wounds
- سال انتشار: 1403
- محل انتشار: یازدهمین کنگره بین المللی زخم و ترمیم بافت یارا
- کد COI اختصاصی: WTRMED11_156
- زبان مقاله: انگلیسی
- تعداد مشاهده: 34
نویسندگان
Shahid Beheshti University
چکیده
Background and purpose: According to the statistics of the Health Organization (۲۰۲۴), diabetic foot ulcer (DFU) includes a high rate of chronic disorders, which has an increasing trend of complications of limb amputation and death, and will have a high economic effect on the health of society. So far, there have been many studies on dressings, now we have designed a hydrogel inspired by the inherent properties of the skin with a rich source of chitosan and hyaluronic acid, which can play an effective role in better tissue regeneration and the extracellular matrix of wounded skin. Materials and methods: In this study, we used chitosan/hyaluronic acid/hydroxypropyl methyl cellulose scaffold network to preparation hydrogel. Also, for better drug delivery, we used silver nanoparticle by lipid transfer system. For animal research, ۸ male Wistar rats of ۲۰۰ grams were used by DFU model with ۱۲ mm diameter, which were divided into two subgroups (n=۴) and evaluated of wound closure rate, SOD enzyme concentration were performed at different times, as well as antibacterial evaluations using the aura of non-growth and non-toxicity was performed on human skin fibroblast cells. In order to check the inherent properties of the hydrogel, water solubility, water vapor transfer, swelling, degradation tests were performed. Results: The results showed that the release rate of silver nanoparticles in the hydrogel was continuous and controlled delivery. In the animal evaluation of the closure of the wound surface, a ۹۵% reduction in the level of damage was observed. In the antioxidant evaluation with the DPPH technique, due to the presence of natural polysaccharides, was observed a decrease in reactive oxygen species, and the antioxidant enzyme concentration had a significant level compared to the control group. In the antibacterial evaluation, a non-growth halo was observed, in the cytotoxicity test by the MTT hydrogel method, ۱۲۶% cell viability and non-toxicity were observed after ۴۸ hours on human skin fibroblast cells. The swelling property of the hydrogel was reported to be ۴۴%. A water vapor transmission rate of ۲۰۲۸ was reported to provide an ideal environment for secretion absorption and cell migration. The degradation rate of the hydrogel also had a good significant level. Conclusion: In the end, it can be said that by strengthening and simulating the inherent properties of hydrogel, inspired byکلیدواژه ها
Hyaluronic Acid, Chitosan, Hydrogel, Wound Dressing, Diabetic Woundsاطلاعات بیشتر در مورد COI
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