Design, Fabrication and In Vivo Pharmacokinetic Study of Coenzyme Q۱۰/Vitamin C-containing Liposomal Drug Delivery System

Background: Systemic inflammatory response syndrome is a chaotic immune response developed by a stressor. Studies show that the prophylactic usage of antioxidant agents, especially correctly combined compositions, can control the underlying biochemical dysregulations and prevent further macrocellular damages. Coenzyme Q۱۰/vitamin C (CoQ۱۰/VitC) composition has been tested successfully against various diseases in which inflammatory response is problematic; however, both drugs are challenged in the terms of pharmacokinetics limiting the clinical effectiveness. Herein, this project aims to resolve these challenges through nanotechnology. Here, CoQ۱۰ / VitC - loaded sunflower - sourced phosphatidylcholine / cholesterol liposomes were designed, fabricated, and tested in vivo. Methods: Optimization and Design: The desired liposomal model was built based on D-optimal mixture design. Additionally, pharmacokinetic parameters were predicted in silico using ADMET prediction software. Quality Control: Final product was studied chemically and thermally via Fourier transform infrared spectroscopy (FT/IR) and Differential scanning calorimetry (DSC), respectively. The morphological properties were assessed using a cryo-transmission electron microscopy (Cryo-TEM). Particle size and zeta potential were determined with a Zeta Sizer Advance. Long-term stability was tested for ۹۰ days at room and ۴۰ oC temperature conditions. Control parameters were as follows: view, physical state, color, odor, density, and viscosity. Cytosafety: Mouse colon adenocarcinoma (C۲۶), hepatocellular carcinoma (HCC۲۸), and primary epidermal keratinocytes (HEKa) cells lines were used for cytosafety assay. PrestoBlue HS was the cell viability reagent. Test plates were studied via BioTek microplate reader with Gen Software. Quantitative Analyses: Quantitative analyses were accomplished via liquid chromatography-mass spectrometry performed