Analysis and preparation of phenolic and alkaloid compounds from Moroccan Berberis vulgaris organic extract: Molecular docking and optimization by UHPLC/DAD/ESI-MS
- سال انتشار: 1403
- محل انتشار: فصلنامه روش های تجزیه در شیمی محیط زیست، دوره: 7، شماره: 2
- کد COI اختصاصی: JR_AMECJ-7-2_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 68
نویسندگان
Laboratory of Sustainable Research Development and Health, Faculty of Sciences and Techniques, University Cadi Ayyad, Marrakech
Department of Chemistry, University of the Free State, P.O. Box ۳۳۹, Bloemfontein ۹۳۰۰, South Africa
Department of Fundamental Sciences, Faculty of Medicine and Pharmacy, and Dentistry of Fez, Sidi Mohamed Ben Abdellah University of Fez, Morocco
Laboratory of Sustainable Research Development and Health, Faculty of Sciences and Techniques, University Cadi Ayyad, Marrakech
Platform chromatographic, Innovation City, University Cadi Ayyad, Marrakech
Platform chromatographic, Innovation City, University Cadi Ayyad, Marrakech
Laboratory of Applied Chemistry and Environment, Faculty of Sciences and Technologies, University Hassan I, Settat
TCPAM, Polydisciplinary Faculty of Sciences University Sultan Moulay Slimane, Beni-mellal
چکیده
Among the natural products, phenolic and alkaloid compounds have the most important constituents and common plant-derived secondary metabolites, and thus, analysis deserves particular attention for their therapeutic proprieties. In this study, the phenolic and alkaloid compounds were analyzed using ultra-high-performance liquid chromatography coupled with diode array detector-tandem electrospray ionization mass spectrometry (UHPLC/DAD/ESI-MS) for three extracts of Moroccan Berberis vulgaris L. Then we optimized and validated results for four phenolic compounds. To evaluate the biological activity of this species we chose the antimicrobial activity. For the antimicrobial activity of these extracts, we used molecular docking analysis of the compounds identified with DNA gyrase. The method validation results showed a good linear (R۲> ۰.۹۹) with the coefficient correlation and the significance of regression tests confirmed a linear relationship between x and y. Two concentrations of phenolic and alkaloid compounds (۳۱.۲۵ mg L-۱ and ۵۰۰ mg L-۱) were determined by the UHPLC/DAD/ESI-MS and used in repeatability tests, the relative standard deviation of ۱۰ repetitions varied to ۰.۳۵-۲.۳۳ with CV% ۱.۱-۲%. In addition, the analytical method is sensitive, accurate, and precise (CV< ۵%). The analytical method successfully set for robust quality evaluation and standardization of phenolic and alkaloid compounds from B. vulgaris organic extract.کلیدواژه ها
analytical method, Alkaloids and phenolic compounds, Ultra-high-performance liquid chromatography, Tandem electrospray ionization mass spectrometry, Berberis Vulgarisاطلاعات بیشتر در مورد COI
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