Platelet-Derived circRNAs hsa_circ_۰۰۰۴۷۷۱ and hsa_circ_۰۰۱۹۱۲۰ Differentially Expressed in Colorectal Cancer and Polyps
- سال انتشار: 1403
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 13، شماره: 3
- کد COI اختصاصی: JR_RBMB-13-3_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 6
نویسندگان
Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
چکیده
Background: Colorectal cancer (CRC) is the second most common cause of cancer-related deaths worldwide. Early detection is crucial for improving survival rates. Liquid biopsies, specifically analyzing circulating tumor-educated platelets (TEPs), have emerged as a promising tool for early CRC detection and monitoring treatment efficacy. This study investigated the expression levels of two specific circRNAs, hsa_circ_۰۰۰۴۷۷۱ and hsa_circ_۰۰۱۹۱۲۰, in the platelets of patients with CRC, advanced polyps, and healthy controls. Methods: Blood samples were obtained from ۲۵ individuals with CRC, ۲۵ individuals with advanced polyps, and ۲۵ healthy controls. Platelet-derived total RNA was extracted, and expression analysis was conducted using reverse transcription quantitative PCR (RT-qPCR). Differential expression and receiver operating characteristic (ROC) curve analysis were performed using GraphPad Prism. Results: Both circRNAs were found to be upregulated in platelets from individuals with advanced polyps and CRC compared to healthy individuals. However, the upregulation was statistically significant only for hsa_circ_۰۰۰۴۷۷۱ in CRC patients (p-value = ۰.۰۰۳۶) and for hsa_circ_۰۰۱۹۱۲۰ in both advanced polyp (p-value = ۰.۰۱۷۵) and CRC patients (p-value = ۰.۰۳۵۶). The combined analysis of both circRNAs achieved an area under the curve (AUC) of ۰.۸۳۴۸ (۹۵% CI: ۰.۷۱۳۱ to ۰.۹۵۶۵) with a sensitivity of ۸۴% and specificity of ۸۰% (p-value = ۰.۰۰۰۲). Conclusions: This study showed that hsa_circ_۰۰۰۴۷۷۱ and hsa_circ_۰۰۱۹۱۲۰ dysregulated in both CRC and polyps and have potential as a novel diagnostic biomarker of CRC.کلیدواژه ها
Biomarker, Blood Platelets, Circular RNA, Colorectal Cancer, Polyps.اطلاعات بیشتر در مورد COI
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