Optimization of Superparamagnetic Iron Oxide Nanoparticles-Trimethyl Chitosan (SPION-TMC) as a siRNA Carrier to Inhibit HIV-۱ nef
- سال انتشار: 1398
- محل انتشار: مجله تحقیقات پاتوبیولوژی، دوره: 23، شماره: 2
- کد COI اختصاصی: JR_PRJMS-23-2_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 72
نویسندگان
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
“Nanotechnology Research Centre” and “Department of Pharmaceutics, Faculty of Pharmacy”, Tehran Uni‐ versity of Medical Sciences, Tehran, Iran
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran
Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Aims: Despite the efficacy of current therapies against HIV-۱ infection, these methods are not a permanent treatment because they cannot prevent the return of viremia from latent cell reservoirs. On the other hand, the virus may become resistant to these drugs. Therefore, providing safer and more effective therapeutic strategies, such as inhibition of genes by siRNA, is essential. The successful therapeutic application of siRNAs requires an efficient delivery system to target cells. Materials & Methods: In this study, a specific siRNA was designed against the HIV-۱ nef gene. Then a stable HEK۲۹۳ cell line expressing HIV-۱ nef was developed and after fabrication and evaluation of superparamagnetic iron oxide nanoparticles (SPION) coated with trimethyl chitosan, the efficiency of nanoparticles for delivering siRNA into the cells and inhibition of nef gene was investigated. Findings: Iron oxide nanoparticles (spherical-shaped with an average size of ۸۵nm and the average zeta potential of +۲۹mV) were significantly effective in transporting siRNA into HEK۲۹۳ cells compared to control groups and at the same time had low toxicity to the cells. In addition, SPION-TMC containing anti-nef siRNA inhibited about ۸۵% of the expression of this gene in stable cells (compared to control cells). Conclusion: The optimized SPION-TMC nanocarriers can be used as a promising approach in HIV-۱ infection therapy. However, pre-clinical in vivo evaluation of the drug/siRNA delivery system efficiency remains to be conducted.کلیدواژه ها
HIV-۱ nef, siRNA Delivery, SPION Carriers, Chitosan, HIV-۱ nef, انتقال siRNA, نانوذرات SPION, کیتوزاناطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.