Evaluation of Lethal Effect of Aqueous and Alcoholic Extracts of Glycyrrhiza glabra on the Emeria tenella Oocysts In Vitro

Aims: Coccidiosis is one of the most common infectious diseases in poultry that causes huge economic losses. Glycyrrhiza glabra is a medicinal plant that used traditionally. The aim of the present study was to evaluate the effect of aqueous and alcoholic extracts of this plant on the Emeria tenella oocysts in vitro. Materials & Methods: The unsporulated oocysts were obtained by inoculation of ۱۴th day-old broiler chicks with ۷۵,۰۰۰ oocysts. To obtain sporulated oocysts, ۹g of feces samples was soaked in ۲% potassium bichromate and incubated at ۲۷°C for ۷۲h. Aqueous and alcoholic extracts of Glycyrrhiza glabra in concentrations of ۱, ۲, and ۵% were prepared and oocysts were exposed to these extracts for ۴۸ hours. Thereafter, the number of sporulated and unsporulated oocysts were counted at ۱, ۱۲, ۲۴, and ۴۸ hours. In order to prevent any error, the experiments were repeated three times. Findings: Both extracts of Glycyrrhiza glabra in all tested concentrations cause a significant reduction in the number of sporulated and unsporulated Eimeria tenella oocysts compared with control (p<۰.۰۵). The rate of inhibitory effect of extracts had a direct relationship with exposure time, and inhibition was continuously increased over time. Conclusion: Alcoholic extracts of Glycyrrhiza glabra had a better effect than aqueous extracts. ۵% alcoholic extract had the best effect. However, further studies are needed to find the best dose for the most anticoccidial effects and also to show its effects on other species of Eimeria and in animal models.

کلیدواژه ها:

Glycyrrhiza glabra ، Sporulated and Unsporulated Oocysts ، Eimeria tenella ، Glycyrrhiza glabra ، اووسیست های اسپوروله و غیر اسپوروله ، آیمریا تنلا ، شرایط آزمایشگاهی