CIVILICA We Respect the Science
(ناشر تخصصی کنفرانسهای کشور / شماره مجوز انتشارات از وزارت فرهنگ و ارشاد اسلامی: ۸۹۷۱)

The Effect of a Co-culture of Mouse Fallopian Tube Cells on Proliferation of Human Endometrial Stem Cells

عنوان مقاله: The Effect of a Co-culture of Mouse Fallopian Tube Cells on Proliferation of Human Endometrial Stem Cells
شناسه ملی مقاله: JR_PRJMS-18-2_001
منتشر شده در در سال 1394
مشخصات نویسندگان مقاله:

شکریه احسانی - Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
مژده صالح نیا - Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
نسیم قربانمهر - Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

خلاصه مقاله:
Objective: This study evaluates the effect of a mouse fallopian tube cell co-culture on the proliferation of human endometrial stromal cells. Methods: We used hysteroscopy to collect the endometrial cells from the uterus. The cells were isolated with collagenase type ۳ and passed through ۱۵۰ µm and ۴۰ µm filters, respectively, after which the isolated cells were cultured in DMEM/F۱۲ medium. At the end of the fourth subculture we used flow cytometry to evaluate the percentage of CD۹۰ positive cells. The endometrial cells were co-cultured with mitomycin C-treated cells from the mouse fallopian tube as the experimental group. Those cultured without the feeder layer were considered the control group. The proliferation rate of cells in both groups were compared by the MTT assay Results: The endometrial cells adhered to the floor of the plate after ۲۴ hours. After ۷۲ hours, they had a spindle shape which was similar to fibroblasts. The rate of CD۹۰ positive cells at the fourth passage was ۹۴.۲۶ ± ۰.۰۸%. The proliferation rate of the co-culture experimental group was ۱.۱ ± ۰.۰۲ and for the control group, it was ۱.۱۷ ± ۰.۱۷ which was not significantly different. Conclusion: Co-culture of mouse fallopian tube cells with endometrial stem cells did not affect the proliferation rate of endometrial stem cells.

کلمات کلیدی:
Co-culture, Human endometrial stem cell, Mouse fallopian tube, سلول های لوله فالوپ موش, سلول های بنیادی آندومتر انسانی, هم کشتی

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/2207352/