ابولفضل دشتی - Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
ملیحه سودی - Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
ناهید امانی - Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Objective: Hexavalent chromium [Cr (VI)] compounds are well-known environmental contaminants generated from industrial processes. Several studies have reported the harmful effects of Cr (VI) on different organs, however, little is known about neurotoxic effects of Cr (VI). The aim of this study is to investigate the toxic effects of Cr (VI) on PC۱۲ cells. Methods: PC۱۲ cells were cultured following standard protocol and exposed to various concentrations (۱-۱۰۰ μM) of potassium dichromate (K۲Cr۲O۷) for ۲۴, ۴۸ and ۷۲ h. After exposure, cell viability was measured by the MTT assy. Also following exposure, production of reactive oxygen species (ROS) and lipid peroxidation were measured. Results: Potassium dichromate induced significant cell death in PC۱۲ cells. The IC۵۰ values for cytotoxicity were ۲۲.۰۲ for ۲۴ h, ۱.۸۸ for ۴۸ h, and ۱.۸۵ for ۷۲ h of exposure. Significant differences between IC۵۰ for ۲۴ h of exposure compared to ۴۸ and ۷۲ h of exposure were observed (p<۰.۰۵). ROS production and lipid peroxidation significantly increased in the Cr (VI) treated groups compared to the control group (p<۰.۰۵). Conclusion: The results indicated that Cr (VI) induced dose and time dependent cytotoxicity in PC۱۲ cells which indicated neurotoxic effects of Cr (VI). Mechanisms of Cr (VI) induced toxicity have not been fully determined, however increased production of ROS and lipid peroxidation in Cr (VI) treated groups demonstrated that oxidative stress might be involved in neurotoxicity of Cr (VI).

Oxidative stress, Cr (VI), Neurotoxicity, کروم VI, سمیت عصبی, استرس اکسیداتیو