Design and Expression of Recombinant HER-۲ Antigen as a Marker for Detection of Breast Cancer
- سال انتشار: 1393
- محل انتشار: مجله تحقیقات پاتوبیولوژی، دوره: 17، شماره: 4
- کد COI اختصاصی: JR_PRJMS-17-4_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 48
نویسندگان
Department of Biology, University of Damghan, Damghan, Iran
Applied biotechnology centre, Baqiyatallah University of Medical Sciences.
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Department of Biology, University of Damghan, Damghan, Iran.
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Objective: The incidence of breast cancer is approximately one million which makes this cancer one of the most common among women worldwide. Breast cancer comprises ۷% of the total death rate caused by cancers. Several strategies that use tumor-associated antigen (TAA) vaccination and early detection of breast cancer are clinically being developed. Breast cancer is caused by increased over expression of certain genes. HER-۲ is a tyrosine kinase receptor in the epidermal growth factor family. The role of HER-۲ in breast cancer has been extensively studied. HER-۲ is found in ۲۵%-۳۰% of breast cancer patients. Herceptin, a human antibody, is used as a therapeutic target for HER-۲. The purpose of this study is to produce recombinant protein HER-۲ for early detection of breast cancer cells. Methods: We used specific primers to amplify the HER-۲ gene. The amplified gene was cloned into pET۲۸a as an expression vector. Cloning was confirmed by restriction analysis and sequencing. Expression was induced using IPTG and the recombinant protein was analyzed by SDS-PAGE. Results: Cloning of the HER-۲ gene was confirmed by enzyme digestion and sequencing. The gene was expressed in E.coli BL۲۱ DE۳. The pET-۲۸a vector which contained the HER-۲ gene showed a high level of expression. The recombinant protein was confirmed by Western blot analysis. Conclusions: A portion of the HER-۲ gene was expressed as a recombinant in E.coli. This could be a good diagnostic test for breast cancer.کلیدواژه ها
Breast Cancer, Cloning and Gene Expression, her-۲ Gene, Recombinant Protein Expression, کلون و بیان ژن, ژن her-۲, تولید نوترکیب پروتئین, سرطان سینهاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.