Evaluation of the Effects of Interferon on Stability of Control Genes in Huh-۷ and HepG۲ Cells
- سال انتشار: 1391
- محل انتشار: مجله تحقیقات پاتوبیولوژی، دوره: 15، شماره: 2
- کد COI اختصاصی: JR_PRJMS-15-2_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 47
نویسندگان
Ph.D. Candidate, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
Assistant Professor, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
Pharmacology and toxicology department, Pharmacology School, University of Tehran, Tehran, Iran
M.Sc., Department of Virology, Pasteur Institute of Iran, Tehran, Iran
چکیده
Objective: Understanding gene expression variations by using RNA transcript analysis methods during hepatic viral protein interactions with the IFN pathway in hepatic cell lines has recently gained importance. One of the most powerful techniques in gene expression quantification is quantitative real-time RT-PCR. Reference genes used as normalizer in this method may be affected across various experimental conditions or treatments. Hence, in the present study, the influence of IFN-a treatment on the mRNA levels of common reference genes including ACTB, GAPDH, TBP, HPRT۱ and HMBS was evaluated in Huh-۷ or HepG۲ cell lines. Methods: Cells were treated with different concentrations of IFN-a. Then, using geNorm and NormFinder programs, we evaluated the expression stabilities of the above prominent reference genes in three sample groups that included each hepatic cell line and the total data sets. Results: HPRT-۱ and GAPDH were the most stable reference genes in the Huh-۷ cell line, whereas ATCB, HMBS and GAPDH were the most stable in the HepG۲ cell line. TBP was one of the least stable reference genes in the three studied groups. Conclusion: This investigation will provide appropriate reference genes for standardization of quantitative real-time PCR data in an IFN-a stimulated model of hepatocyte cell lines.کلیدواژه ها
Huh-۷, HepG۲, IFN-, Huh-۷, HepG۲, Control Genes, اینترفرون آلفا, ژنهای کنترلاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.