The impact of Polymorphisms of rs۶۸۹ and rs۷۵۷۱۱۰ in Familial Type ۲ Diabetes: A Case-Control Study in the Iranian Population
- سال انتشار: 1403
- محل انتشار: مجله دیابت و چاقی ایران، دوره: 17، شماره: 1
- کد COI اختصاصی: JR_IJDO-17-1_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 19
نویسندگان
Department of Biology, Faculty of Engineering and Science, Science and Arts University, Yazd, Iran.
Department of Biology, Faculty of Engineering and Science, Science and Arts University, Yazd, Iran.
Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mohammad Yahya Vahidi Mehrjardi
Diabetes Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
چکیده
Objective: Understanding the genetic basis of type ۲ diabetes mellitus (T۲DM) is essential in getting its etiology and designing effective preventive strategies. Evaluation of the association between the gene polymorphisms rs۶۸۹A/T (INS) and rs۷۵۷۱۱۰C/A (ABCC۸) and the susceptibility to T۲DM within a group of individuals diagnosed with T۲DM in Iran. Materials and Methods: Blood samples were used for DNA extraction (۲۰۰ with T۲DM and ۲۰۰ healthy controls). The quality and quantity of extracted DNA were assessed via ultraviolet spectrophotometry at ۲۶۰ nm and ۲۸۰ nm wavelengths. To identify specific alleles, primer sequences were manually designed using Primer۳Plus, and the genotypes of rs۶۸۹ (A> T) and rs۷۵۷۱۱۰ C> A were determined through ARMS-PCR. Statistical analysis was conducted using GraphPad Prism version ۸. Results: For rs۶۸۹ individuals with the genotype (AA) were found to have a higher likelihood of developing diabetes (P= ۰.۰۰۱). Additionally, the frequency of the A allele was found to be higher in the patient group (۰.۱۳) compared to the control group (۰.۰۵). For rs۷۵۷۱۱۰ individuals with the genotype (CC) were found to have a higher likelihood of developing diabetes (P< ۰.۰۰۱). Additionally, the frequency of the C allele was found to be higher in the patient group (۰.۵۷) compared to the control group (۰.۳۹). Conclusion: This study found that the frequency of AA and AT genotypes of rs۶۸۹ and CC and CA genotypes of rs۷۵۷۱۱۰ are associated with T۲DM risk.کلیدواژه ها
Diabetes, ARMS-PCR, rs۶۸۹, rs۷۵۷۱۱۰اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.