Alpha-pinene ameliorates liver fibrosis by suppressing oxidative stress, inflammation, and the TGF-β/Smad۳ signaling pathway
- سال انتشار: 1404
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 28، شماره: 4
- کد COI اختصاصی: JR_IJBMS-28-4_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 110
نویسندگان
Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Department of Biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
چکیده
Objective(s): A monoterpene alpha-pinene possesses anti-oxidant, anti-inflammatory, and anti-apoptotic properties. Here, we investigated the effect of alpha-pinene on molecular, biochemical, and histological changes induced by carbon tetrachloride (CCl۴) in the liver of male Wistar rats. Materials and Methods: Animals were divided into four groups: Control, Pinene, CCl۴, and CCl۴.Pinene. Pinene and CCl۴.Pinene groups were given alpha-pinene (۵۰ mg/kg/day) through intraperitoneal (IP) injections for six consecutive weeks. CCl۴ and CCl۴.Pinene groups received IP injections of CCl۴ (۲ ml/kg twice weekly for six consecutive weeks).Results: The results revealed that alpha-pinene inhibited enhancing liver enzyme AST (P< ۰.۰۰۱), ALT (P< ۰.۰۰۱), ALP (P< ۰.۰۱), and GGT (P< ۰.۰۰۱) activity in CCl۴.Pinene rats. It reduced malondialdehyde (P< ۰.۰۵) and nitric oxide (P< ۰.۰۵) levels and increased the catalase enzyme activity (P< ۰.۰۵) and glutathione levels (P< ۰.۰۱) in the liver. Likewise, alpha-pinene suppressed proinflammatory and profibrotic gene expression and prevented significant histological damage and collagen deposition in the liver of these animals. Also, alpha-pinene reduced the expression of TLR۴ (P< ۰.۰۱), NF-κB (P< ۰.۰۵), PI۳K (P< ۰.۰۵), Akt (P< ۰.۰۵), mTOR (P< ۰.۰۱), TGF-β۱ (P< ۰.۰۱), and Smad۳ (P< ۰.۰۱) in the liver of rats receiving CCl۴. Conclusion: We concluded that alpha-pinene reduced CCl۴-induced liver fibrosis by lowering oxidative stress, suppressing liver inflammation, and inhibiting TLR۴/NF-κB, TGF-β/Smad۳, and PI۳K/Akt/mTOR signaling pathways. Consequently, alpha-pinene may have potential therapeutic value in treating liver diseases.کلیدواژه ها
Alpha-pinene, Carbon tetrachloride, Collagen, Glutathione, Inflammation, Toll-like receptor ۴اطلاعات بیشتر در مورد COI
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