In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles
- سال انتشار: 1404
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 28، شماره: 4
- کد COI اختصاصی: JR_IJBMS-28-4_012
- زبان مقاله: انگلیسی
- تعداد مشاهده: 80
نویسندگان
Institute of Police Equipment and Technologies, Policing Sciences and Social Studies Research Institute, Tehran, Iran
Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Research Center for Life & Health Sciences & Biotechnology of the Police, Directorate of Health, Rescue & Treatment, Police Headquarters, Tehran, Iran
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Department of Radiology Technology, School of Paramedical Sciences, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran
چکیده
Objective(s): Due to its low toxicity and high absorbance in the range of ۶۵۰ to ۹۰۰ nm, indocyanine green (ICG) has garnered significant attention for its applications in photodynamic therapy (PDT) and photothermal therapy (PTT). However, its tendency to aggregate in aqueous environments limits its efficacy in both in vitro and in vivo applications. Encapsulating ICG in a biocompatible nanomicelle can improve its aqueous stability and photophysical properties. The present study investigated the synergistic effect of ICG-loaded nanomicelles upon irradiation by an ۸۰۸-nm laser on Leishmania major (L. major) parasites. Materials and Methods: Initially, a nanomicelle comprised ICG was synthesized and characterized. Then, the temperature increase during irradiation and promastigote viability were evaluated in vitro. Subsequently, the prepared samples’ in vitro dark toxicity and phototoxicity were assessed via the MTS assay. Finally, the in vivo antileishmanial efficacy of the ICG-loaded nanomicelles formulation was investigated in BALB/c mice. Results: The absorbance of ICG-loaded nanomicelles at ۸۰۸ nm was more than ۲ times greater than Free-ICG. Also, the prepared formulation exhibited a mean diameter of ~۲۵ nm and a zeta potential of -۲.۳ ± ۱ mV. The combination of ICG-loaded nanomicelles and ۸۰۸ nm laser with a power density of ۲.۵ W cm−۲ led to a significant reduction in the survival rate of promastigotes and lesion size of infected mice compared to control groups. Conclusion: The PDT/PTT mediated by ICG-loaded nanomicelles can be considered a promising and efficient therapeutic method for L. major, as it is inexpensive, safe, and easy to implement.کلیدواژه ها
Leishmania major, Nanomedicine, Photodynamic Therapy (PDT), Photosensitizer, Photothermal therapy (PTT)اطلاعات بیشتر در مورد COI
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