Investigation of Non-Coding RNA-Related Autophagy Alterations in Drug-Resistant Multiple Myeloma Plasma Cells
- سال انتشار: 1403
- محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 19، شماره: 4
- کد COI اختصاصی: JR_IJP-19-4_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 16
نویسندگان
Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Thalassemia & Hemoglobinopathy Research center, Health research institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Thalassemia & Hemoglobinopathy Research center, Health research institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Thalassemia & Hemoglobinopathy Research center, Health research institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Background & Objective: Multiple myeloma (MM) drug resistance is thought to be caused by the development of protective autophagy. This work aimed to assess the non-coding RNA (ncRNA) autophagy-related alterations in drug-resistant (DR) myeloma cells.Methods: DR Plasma cells were extracted from the bone marrow of DR patients referred to Baghai ۲ Hospital in Ahvaz, Iran. The cells were grown in RPMI-۱۶۴۰ media containing ۱۰% FBS and ۱% Pen/Strep and incubated at ۳۷˚C and ۵% CO۲. After six passages, the plasma cells were precisely isolated and utilized as DR cells. The U۲۶۶B۱ cell line (IBRC C۱۰۱۴۸) was grown in the RPMI-۱۶۴۰ media containing ۱۰% FBS and ۱% Pen/Strep and utilized as drug-sensitive (DS) cells. The relative expression of the genes was determined using the Real-time PCR method. Statistical analysis of the data was performed using GraphPad Prism ۸ software. Results: When the DR cells were compared to the DS cells, there was a notable increase in the expression of ULK۱ and LC۳B. However, expression of P۶۲ in the DR plasma cells showed a significant decrease compared to the DS plasma cells. The miR-۱۲۹۷ level was considerably higher in the DR cells than in the DS cells. Although, there was no statistically significant difference in the expression of miR-۲۶a-۵p between the DS and DR cells. The DR cells exhibited a statistically significant increase in the expression of MALAT۱ and SNHG۶.Conclusion: Drug resistance in MM cells may result from overexpression of non-coding RNAs involved in autophagy.کلیدواژه ها
Multiple Myeloma, Drug resistance, autophagy, non-coding RNA, Plasma cellاطلاعات بیشتر در مورد COI
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