Puerarin alleviates renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via Nrf۲/HO-۱ pathway
- سال انتشار: 1404
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 28، شماره: 2
- کد COI اختصاصی: JR_IJBMS-28-2_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 131
نویسندگان
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, ۴۳۰۰۶۰, China
چکیده
Objective(s): To explore the effects of puerarin on renal ischemia/reperfusion injury and the possible mechanism.Materials and Methods: The experimental mice were injected with puerarin (۵۰ or ۱۰۰ mg/kg) per day or equal sterile saline by intraperitoneal injection for one week, and a renal I/R injury model was constructed. HK-۲ cells were incubated with puerarin (۱ uM and ۱۰ uM) before the H/R model. Immunohistochemistry, immunocytochemistry, and Western blot analysis were used to detect the protein associated with apoptosis and endoplasmic reticulum stress.Results: Puerarin could improve renal function and attenuate tissue structural damage after renal I/R. Meanwhile, puerarin alleviated apoptosis and endoplasmic reticulum stress by decreasing expression levels of specific biomarkers such as caspase-۳, GRP۷۸, CHOP, and p-elF۲α/ elF۲α in animals and HK-۲ cells. The up-regulated expression of Nrf۲ and HO-۱ protein after puerarin treatment indicated that the Nrf۲/HO-۱ signaling pathway might mediate the protective mechanism of puerarin against renal I/R.Conclusion: Our results suggest that puerarin alleviated renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via the Nrf۲/HO-۱ pathway and offered new insights for preventing and treating renal I/R.کلیدواژه ها
Apoptosis, Endoplasmic reticulum- stress, Nrf۲/HO-۱ pathway, Puerarin, Renal ischemia/reperfusion- injuryاطلاعات بیشتر در مورد COI
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