The promotor methylation level of ATG۱۲ correlates with tumor progression and autophagy in Bladder cancer; A bioinformatic study

  • سال انتشار: 1402
  • محل انتشار: دوازدهمین همایش ملی و سومین همایش بین المللی بیوانفورماتیک
  • کد COI اختصاصی: IBIS12_085
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 121
دانلود فایل این مقاله

نویسندگان

Mir Shayan Jahangirzadeh

Department of Biology, Faculty of Basic Science, Azarbaijan Shahid Madani University, Tabriz, Iran

Mehdi Asghari Vostakolaei

Cancer Immunology and Immunotherapy Research Center, Ardabil University of Medical Sciences, Ardabil, Iran

Asadollah Asadi

Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran

Masoumeh Valipour

Department of Biology, Faculty of Basic Science, Azarbaijan Shahid Madani University, Tabriz, Iran

چکیده

Bladder cancer (BC) is among the top ten most common cancer types in the world [۱]. Theheterogeneity of BC calls for substantial research with more in-depth molecular characterization, withthe hope of identifying new diagnostic and treatment options [۲]. DNA methylation is an epigeneticmechanism involving the transfer of a methyl group. DNA methylation regulates gene expression byrecruiting different proteins or by inhibiting the binding of transcription factors to DNA. Methylationcan alter gene expression and regulate different signaling pathways such as autophagy [۳]. Autophagyhas a conserved mechanism whose main role is to recycle cellular components and maintainhomeostasis through the removal of undesirable proteins [۴]. ATG۱۲ is one of the most important genesinvolved in autophagy pathways [۵]. This study aimed to explore the function of the ATG۱۲ gene in theautophagy pathway involved in BC based on bioinformatic databases. Clinical information andpromotor methylation level of ATG۱۲ in patients with BC was obtained from The Cancer Genome Atlas(TCGA) based on the following selection criteria including basic clinical information of sample types(normal and primary tumor), gender, age, stage, and nodal metastasis. All requirements were conductedon a large sample size (> ۴۰۰). Datasets were then compared to obtain the significant p-value. Our resultsindicate that the promotor methylation level of ATG۱۲ is decreased in BC compared with normal tissue.This decrease was observed in different BC stages (۱-۴), different age groups (۲۱-۴۰, ۴۱-۶۰, ۶۱-۸۰, and۸۱-۱۰۰ ys), gender, and nodal metastasis status of BC. There were no significant differences in genderand nodal metastasis (N۰, N۱, N۲, and N۳) groups. Our study revealed that ATG۱۲ can be consideredan important factor in BC progress with diagnostic and prognostic values.

کلیدواژه ها

ATG۱۲; Bladder cancer; Methylation; Autophagy; The Cancer Genome Atlas (TCGA)

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.