Stereological and biochemical effects of thymoquinone on ovarian tissue toxicity induced by silver nanoparticles in NMRI mice: An experimental study
- سال انتشار: 1403
- محل انتشار: International Journal of Reproductive BioMedicine، دوره: 22، شماره: 7
- کد COI اختصاصی: JR_IJRM-22-7_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 489
نویسندگان
Seyed Mohammad Ali Shariatzadeh
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
چکیده
Background: The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of Nigella sativa. Objective: We evaluated the efficacy of TQ on ovarian tissue following toxicity induced by AgNPs in female mice. Materials and Methods: ۲۴ female NMRI mice (۵-۶ wk, an average weight of ۳۳ gr) were randomly divided into ۴ groups (n = ۶/each): control, AgNPs (۵۰۰ mg/kg, gavage), TQ (۲.۵ mg/kg, intraperitoneal injection), and TQ+AgNPs. Mice were treated every day for ۳۵ days. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), luteinizing hormone, and follicle-stimulating hormone were measured. The optical disector and stereological techniques were utilized to estimate the follicular count, their volume at different developmental stages, and the structure of ovarian tissue. Results: In the AgNPs group, the serum concentrations of TAC (p = ۰.۰۱), luteinizing hormone (p < ۰.۰۰۱), follicle-stimulating hormone, the volume of corpus luteum (p < ۰.۰۰۱), and the number of follicles decreased significantly compared to the control group. Nevertheless, AgNPs significantly increased the MDA level. In the TQ+AgNPs group compared to the AgNPs group, a significant decrease in MDA level (p < ۰.۰۰۱) and a significant improvement in TAC (p = ۰.۰۳), and hormonal levels, the number of primary, preantral, and antral follicles (p = ۰.۰۴), and the volume of corpus luteum (p = ۰.۰۱) were observed. Conclusion: TQ improved the number of follicles by reducing oxidative stress and lipid peroxidation in AgNPs-damaged ovarian tissue.کلیدواژه ها
Nanoparticles, Silver, Thymoquinone, Ovary, Mouse., نانوذرات, نقره, تیموکینون, تخمدان, موش.اطلاعات بیشتر در مورد COI
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