Impact of chronic opioid on cognitive function and spermatogenesis in rat: An experimental study

  • سال انتشار: 1403
  • محل انتشار: International Journal of Reproductive BioMedicine، دوره: 22، شماره: 7
  • کد COI اختصاصی: JR_IJRM-22-7_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 170
دانلود فایل این مقاله

نویسندگان

Hamid Norioun

Medical Genetics Department, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Seyed Jamal Moshtaghian

Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.

Firoozeh Alavian

Department of Biology, School of Basic Sciences, Farhangian University, Tehran, Iran.

Maryam Khombi Shooshtari

Chronic Renal Failure Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Golnaz Alipour

Microbiology Department, Medical Faculty, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Saeedeh Ghiasvand

Biology Department, Faculty of Sciences, University of Malayer, Hamedan, Iran

چکیده

Background: Opioid analgesics like morphine and methadone are widely used for managing severe pain; however, concerns over their potential misuse and adverse effects on the brain and reproductive system are significant. Objective: We aimed to investigate their impacts on spermatogenesis and cognitive function in male Norway rats. Materials and Methods: In this experimental study, ۳۶ male Norway rats (۲۵۰-۳۰۰ gr, ۶ months old) were divided into ۶ groups: low-dose morphine, high-dose morphine, low-dose methadone, high-dose methadone, positive control (received normal saline at ۵ mg/kg), and negative control (received no treatment). Morphine and methadone were administered intraperitoneally over ۳۰ days at doses of ۳ mg/kg and ۷ mg/kg, respectively. Behavioral assessments evaluated anxiety, stress, and short- and long-term memory. Sperm parameters (viability, motility, morphology), hormonal analysis (testosterone, luteinizing hormone, follicle-stimulating hormone, estradiol), and gene expressions (Tp۵۳, CatSper۱) were assessed. Results: A significant reduction in rat weight was observed in the high-dose morphine group (p = ۰.۰۰۴۵), while testicular weights remained unchanged. Sperm abnormalities were observed with high doses of methadone and morphine. High-dose methadone significantly reduced offspring count (p = ۰.۰۰۰۴). Levels of follicle-stimulating hormone, luteinizing hormone, testosterone, and estradiol varied significantly across treatment groups. Gene expression was altered in response to treatments (p < ۰.۰۵). Conclusion: Prolonged exposure to methadone and morphine resulted in memory dysfunction, chronic stress, hormonal disturbances, altered gene expression, and fertility complications. These effects were more pronounced at higher doses, highlighting the importance of careful dosage management in opioid therapy.

کلیدواژه ها

Rat, Spermatogenesis, CatSper۱, Tp۵۳, Morphine, Methadone, Cognition., موش, اسپرماتوژنز, CatSper۱, TP۵۳, مورفین, متادون, حافظه.

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.