Plasmid-mediated colistin and fosfomycin resistance among clinical isolates of ESBL- and carbapenemase-producing Klebsiella pneumoniae in Northern Iran
- سال انتشار: 1403
- محل انتشار: مجله آرشیو رازی، دوره: 79، شماره: 4
- کد COI اختصاصی: JR_ARCHRAZI-79-4_023
- زبان مقاله: انگلیسی
- تعداد مشاهده: 196
نویسندگان
Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Noncommunicable diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Noncommunicable diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Seyyed Khalil Shokouhi Mostafavi
Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Noncommunicable diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
چکیده
The development of extensively-resistant strains of Klebsiella pneumoniae (K. pneumoniae) in the hospitals is associated with hospitalization and uncontrolled use of antibiotics. There is a scarcity in the colistin and fosfomycin resistance encoding genes rate and mechanisms in Iran. Our aim was to determine the rate of biofilm formation and fosfomycin and colistin resistance among K. pneumoniae producing ESBL and carbapenemases by detection of mcr-۱, mcr-۲ and fosA genes in Tehran, Iran during ۲۰۲۰-۲۰۲۱. After collecting ۷۳ samples, the isolates were identified using biochemical tests. The antibiotic susceptibility test was implemented using disk diffusion method. The phenotypic determination of extended-spectrum beta-lactamases (ESBLs) and carbapenemase enzymes was conducted using combined disk and CARBA-NP tests, respectively. The biofilm formation was performed using microtiter tissue plate assay. The polymerase chain reaction (PCR) was performed to detect mcr-۱, mcr-۲ and fosA genes associated with colistin and fosfomycin resistance, respectively. The highest resistance rate was against ampicillin (۹۷%), chloramphenicol (۹۰%) and ciprofloxacin (۸۷%), respectively, while the lowest resistance rate was against gentamicin (۴%), amikacin (۱۰%), and cotrimoxazole (۱۸%). Moreover, ۴۴ and ۲۳ isolates were ESBL and carbapenemase producing K. pneumonia), respectively. Forty-eight isolates were strong biofilm forming K. pneumoniae and one isolate was non-biofilm producer. In the PCR test, four isolates amplified the fosA۲ and one mcr-۲ genes, respectively, while none of them outlined neither fosA۳ nor mcr-۱ genes amplification. This study demonstrated that the frequency of K. pneumoniae isolates producing ESBL and carbapenemase and also mcr-۱, mcr-۲ and fosA genes was low but considering facilitated spread of these genes, proper isolation and control is essential. Moreover, these strains had the ability to form biofilms in vitro which persist infections in the hospital settings.کلیدواژه ها
Klebsiella pneumoniae, Antibiotic resistance, Colistin, ESBL, carbapenemaseاطلاعات بیشتر در مورد COI
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