The Effect of in Ovo Serotonin on Liver Hsp۷۰ and Hsp۹۰ Gene Expression in Heat Stressed Broiler Embryo

High environmental temperatures and heat stress negatively impact physiological performance. Because chicks lack sweat glands, they are particularly sensitive to heat stress, making them a valuable model for studying this phenomenon. Serotonin (۵-hydroxytryptamine, ۵-HT) also plays a vital role in stress responses across various animal species, including birds, influencing their physiological and metabolic adaptations to challenging conditions. Heat shock proteins (Hsps) form a group of proteins synthesized in response to different stressors, safeguarding cells from harm. This study aimed to explore the impact of serotonin (۲۰ micrograms per egg) on the expression of Hsp۷۰ and Hsp ۹۰ genes after exposure to high temperatures (۳۹.۵°C) for varying durations (۲, ۴, ۶, ۸, and ۱۰ hours). Data analysis showed no significant changes in Hsp۷۰ gene expression in embryonic liver tissue among different groups. Similarly, Hsp ۹۰ gene expression remained unchanged in the heat stress group compared to the control group. However, serotonin led to a notable increase in Hsp ۹۰ gene expression in the heat stress condition group. These findings, observed in Ross broiler embryos, suggest alterations in Hsp ۹۰ gene expression in the liver under heat stress conditions and highlight serotonin's role as an anti-stress agent stimulating Hsp gene expression. These results imply the potential utilization of nutritional supplements in the poultry industry to regulate Hsps, particularly in situations of heat stress.

کلیدواژه ها:

Heat stress ، serotonin ، Hsps ، liver ، chicken embryo