Comparison of Cattle Serum Antibody Responses to Five Different Mycobacterial Antigens in an ELISA System
- سال انتشار: 1403
- محل انتشار: دومین کنگره بین المللی علوم، مهندسی و فن آوری های نو
- کد COI اختصاصی: SECONGRESS02_246
- زبان مقاله: انگلیسی
- تعداد مشاهده: 204
نویسندگان
Department of Virology Vaccines, Production and Research Unit, Pasteur Institute of Iran, Karaj, Iran
Department of Microbiology, Faculty of Basic Sciences, Shahrekord Azad University, Shahrekord, Iran
Department of Microbiology, Faculty of Basic Sciences, Shahrekord Azad University, Shahrekord, Iran
Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Department of Food and Drug Control, Zanjan University of Medical Sciences, Zanjan, Iran
Seyed Mohammad Ghorbani Dazmiri
Faculty of Biological Sciences, Sari Branch, Islamic Azad University, Sari, Iran
چکیده
The presence of common zoonosis diseases caused by Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM), such as Johne's and Crohn's diseases, poses a public health threat and economic losses to Iranian livestock. Therefore, the early detection of mycobacteria is of paramount importance. In this regard, enzyme-linked immunosorbent assay (ELISA) is a new, simple to use, rapid, and useful diagnostic tool. This study was performed to evaluate different crude antigens obtained from Mycobacterium species using an indirect ELISA test to identify the mycobacterial infection in infected livestock. Five different strains of Mycobacteria including M. tuberculosis, M. phlei, M. bovis, M. avium subspecies paratuberculosis, and M. bovis AN۵ were cultured. The crude antigens in the samples were precipitated with trichloroacetic acid ۴%. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis of crude antigens isolated from different Mycobacterium species was reported. The total level of protein was determined by the Lowry protein assay. After the crude antigen preparation, the ELISA test was performed and the results were compared with the purified protein derivative skin test. Data analysis was performed using SPSS software version ۲۵. All five strains were detected in more than ۹۲% of healthy animals. The highest sensitivity of ELISA tests was in M. bovis AN۵ antigen which was greater than ۸۳%. The highest diagnostic specificity and efficiency of assays were in M. avium subspecies paratuberculosis which was ۹۵.۸۳% and over ۸۳%, respectively. Regarding the results, M. avium subspecies paratuberculosis and M. bovis AN۵ antigens were promising candidates for the design of diagnostic ELISA due to their sensitivity, specificity, and efficiency.کلیدواژه ها
Mycobacteria, Nontuberculous mycobacteria, Purified protein derivative test, Enzyme-linked immunosorbent assayاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.