The Effects of Folic Acid on Testicular Histology, Sperm Quality, and Spermatogenesis Indices Following ۳,۴-Methylenedioxymethamphetamine Exposure in Adult Male Rats

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 81

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شناسه ملی سند علمی:

JR_AHJK-13-1_005

تاریخ نمایه سازی: 9 مرداد 1403

چکیده مقاله:

Background: There is an increasing concern over acute exposure of amphetamine and its derivative such as۳,۴-methylenedioxymethamphetamine (MDMA) on male reproductive toxicity. Supplementary vitamins canreduce the oxidative stresses and repair the damages on reproductive organs. This experimental study wasconducted to evaluate the effects of folic acid (FA) on reproductive indices, the antioxidant enzyme activities,and histological changes of testis on adult male rats treated by MDMA.Methods: This experimental study was conducted on adult male Wistar rats. Animals were divided into ۴groups: control, MDMA, FA, and MDMA + FA. Animals received a dose of ۱۰ mg/kg of MDMA and ۱ mg/kg ofFA for ۷ or ۱۴ days. Rats were anesthetized and sperm quality parameters (number, concentration, motility,and morphology), spermatogenesis indices [the mean seminiferous tubule diameter (MSTD), spermiogenesisindex (SI), repopulation index (RI), and tubular differentiation index (TDI)], changes on testicular structure,antioxidant enzyme activities of catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA)beside serum level of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone weremeasured. Data were analyzed, using the one-way analysis of variance (ANOVA) and SPSS software.Findings: MDMA (both ۷ and ۱۴ days) caused significant changes in sperm quality (P < ۰.۰۰۱),spermatogenesis indices (P < ۰.۰۰۱), testicular histopathology, and level of LH, FSH, testosterone beside theantioxidant enzyme activities (SOD, CAT, and MDA) (P < ۰.۰۰۱). Supplementation of FA in association withMDMA partially reversed these parameters and made them close to the control group.Conclusion: The results suggested that FA could reduce the adverse effect of MDMA on reproductive ability inadult male rats.

نویسندگان

Iman Rad

Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Arezoo Saberi

Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Najmeh Sadat Koochakzadeh-Nematollahi

Afzalipour Clinical Center for Infertility, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran

Victoria Habibzadeh

Afzalipour Clinical Center for Infertility, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran

Ehsan Salarkia

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

Sedigheh Amanollahi

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

Saeideh Nikpour

Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran