Opium Induces Apoptosis in Jurkat Cells

  • سال انتشار: 1392
  • محل انتشار: مجله اعتیاد و سلامت، دوره: 5، شماره: 1
  • کد COI اختصاصی: JR_AHJK-5-1_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 160
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نویسندگان

Somayeh Igder

MSc Student, Department of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, Iran

Gholam-Reza Asadikaram

Professor, Physiology Research Center AND Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Farzaneh Sheykholeslam

Assistant Professor, Pasture Institute, Tehran, Iran

Ahmad-Reza Sayadi

Department of Psychology, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, Iran

Mehdi Mahmoodi

Professor, Department of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, Iran

Mohammad Kazemi Arababadi

Assistant Professor, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, Iran

Mohammad Javad Rasaee

Professor, Department of Biotechnology, School of Medicine, Tarbiat Modares University, Tehran, Iran

چکیده

Background: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. Methods: Different concentrations of opium (۲.۸۶ × ۱۰-۳ to ۲.۸۶ × ۱۰-۱۱ g/ml) were added to ۲۴-well plates containing ۵ × ۱۰۵ Jurkat cells. Apoptotic events were assessed after ۶, ۲۴, and ۷۲ hours by flow-cytometric detection of surface phosphatidylserine. Findings: Significant differences in apoptosis of Jurkat cells were seen at ۲۴ and ۷۲ hours in different concentrations of opium (P < ۰.۰۵). After ۷۲ hours, significant increase in necrosis of Jurkat cells was seen in opium concentration of ۲.۸۵ × ۱۰-۳ g/ml compared to cells without opium (control) (P < ۰.۰۵). Conclusion: These results showed that opium directly increases apoptosis and necrosis of T lymphocytes. This effect may play a role in immune dysfunction in opium addicts.Keywords: Opium, Apoptosis, Necrosis, Jurkat cells

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