(-)-α-Bisabolol inhibits D-Gal-induced HSF cellular senescence in vitro and prevents skin aging in vivo by reducing SASP
- سال انتشار: 1403
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 27، شماره: 9
- کد COI اختصاصی: JR_IJBMS-27-9_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 111
نویسندگان
The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China
The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China
Research and Development Department, Guangzhou Qinglan Biotechnology Company Limited, Guangzhou, China
The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China
The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China
Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, China
Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, China
State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
چکیده
Objective(s): To study the anti-aging effect of (-)-α-bisabolol ((-)-α-bis) on the skin and preliminarily clarify its mechanism. Materials and Methods: Human skin fibroblasts (HSF) were induced senescence by D-Galactose. Senescence β-galactosidase staining was utilized to evaluate the senescence of HSF. TNF-α, IL-۶, IL-۸, IL-۱β, CCL-۲, CCL-۵, and MMP-۹ in senescence-as-sociated secretory phenotype (SASP) were detected by RT-qPCR. Meanwhile, aged BALB/c mice were applied topically with ۰.۵% and ۲%(-)-α-bis gel for ۳۰ days continuously to evaluate anti-aging parameters on the skin such as surface measurement, the Trans Epidermal Water Loss (TEWL), and skin barrier index of dorsal skin. Then, HE staining, Masson staining, and IHC were applied to measure epidermal thickness, collagen fiber content in the dermis, and content of dermal collagen I, respectively. Last, SOD, MDA, and HYP contents of the back skin tissue of mice were also detected. Results: (-)-α-Bis reduced the expression of senescence-associated β-galactosidase (SA-β-gal) and expression levels of SASP in HSF cells stimulated by D-Gal (P< ۰.۰۵). Mice aged ۹ months were applied locally with (-)-α-bis gel to improve skin aging, the TEWL and skin barrier index of dorsal skin, and ameliorate the epidermal thickness and contents of dermal collagen fibers and collagen I (P< ۰.۰۵). Furthermore, (-)-α-bis up-regulated the mRNA expression levels of elastin and collagen III effectively (P< ۰.۰۵). Conclusion: (-)-α-Bis can delay the senescence of HSF cells by reducing the expression of SA-β-gal and SASP factors in vitro. Improved skin barrier function as well as SASP is responsible for the delay of skin aging in vivo.کلیدواژه ها
(-)-α-Bisabolol, Cellular senescence, D-galactose, SASP, Skin agingاطلاعات بیشتر در مورد COI
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