Injectable multifunctional hydrogel containing Sphingosine ۱-phosphate and human acellular amniotic membrane for skin wound healing
- سال انتشار: 1403
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 27، شماره: 9
- کد COI اختصاصی: JR_IJBMS-27-9_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 149
نویسندگان
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Mechanical Engineering, New Jersey Institute of Technology, New Jersey, United States of America (USA)
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
چکیده
Objective(s): The skin serves as the main defense barrier, protecting against injuries, and preventing infection and water loss. Consequently, wound healing and skin regeneration are crucial aspects of wound management. A novel hydrogel scaffold was developed by incorporating carboxymethyl cellulose (CMC) and gelatin (Gel) hydrogels cross-linked with ۱-(۳-dimethylaminopropyl)-۳-ethylcarbodiimide hydrochloride (EDC) containing Sphingosine ۱-phosphate (S۱P). This hydrogel is applied topically to treat acute wounds and is covered with a human acellular amniotic membrane (hAAM) as a secondary dressing. Materials and Methods: The scaffold was subjected to in vitro cell viability, red blood cell hemolysis, blood clotting index, and in vivo assays. Real-time PCR was implemented to verify the expression of genes involved in skin wounds. The physical and chemical properties of the scaffolds were also tested using weight loss, swelling ratio, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and mechanical tensile analysis. Results: The synthetic scaffold is biocompatible as evidenced by the high percentage of ۳T۳ cell viability (۱۲۷%) after ۷۲ hr. Additionally, excellent hemocompatibility with a low hemolytic effect (۲.۲۶%) was observed. Our in vivo wound healing assay demonstrated that CMC/Gel/S۱P/hAAM wound dressing led to faster wound healing in treated rats compared to the control group over ۱۴.Also, the mechanical tests showed that the amniotic membrane and the hAAM had very different Young’s modulus and elongation at break values.Conclusion: This study demonstrates the effectiveness of the CMC/Gel/EDC hydrogel with S۱P as a wound dressing. Additionally, hAAM exhibits excellent characteristics as a protective layer for the treatment of acute wounds.کلیدواژه ها
Amnion, Gelatin, Hydrogels, Sodium carboxymethylcellulose, Sphingosine ۱-phosphate, Wound healingاطلاعات بیشتر در مورد COI
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