Serum miR-۲۳ and miR-۱۵۰ Profiles as Biomarkers for Predicting Recurrence following Surgical Intervention in Colorectal Cancer Patients
- سال انتشار: 1402
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 12، شماره: 4
- کد COI اختصاصی: JR_RBMB-12-4_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 215
نویسندگان
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey.
Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey.
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
چکیده
Background: MicroRNAs (miRNAs) play pivotal roles in post-transcriptional regulation of gene expression and have emerged as crucial regulators in cancer development, progression, and metastasis. This study aimed to assess the expression profiles of miR-۲۳, miR-۲۲۳, miR-۱۲۴۶, and miR-۱۵۰ in serum samples obtained from colorectal cancer (CRC) patients before and three months after surgery, in comparison to a healthy control group, to explore their biomarker potential. Methods: A total of ۵۰ blood samples were collected from patients with CRC (pre- and post-surgery), along with ۵۰ samples from healthy controls. The relative expression levels of miR-۲۳, miR-۲۲۳, miR-۱۲۴۶, and miR-۱۵۰ in the serum were quantified using quantitative real-time PCR. Results: Our findings revealed upregulated expression levels of miR-۲۳, miR-۱۲۴۶, and miR-۲۲۳, while miR-۱۵۰ exhibited significant downregulation in the serum of CRC subjects compared to healthy controls. Receiver operating characteristic (ROC) analysis indicated that miR-۲۳ and miR-۱۵۰ could distinguish CRC cases from controls with relatively high accuracy. Moreover, three months post-surgery, miR-۲۳, miR-۱۲۴۶, and miR-۲۲۳ serum levels were downregulated, and miR-۱۵۰ was significantly upregulated. However, no significant correlations were observed between serum levels of the studied genes and the clinical features of our patients. Conclusions: The serum levels of miR-۲۳ and miR-۱۵۰ hold promise as potential biomarkers for the diagnosis and prognosis of CRC.کلیدواژه ها
Biomarker, Colorectal cancer, micro-RNAs, Tumorigenesis.اطلاعات بیشتر در مورد COI
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