Correlation of MicroRNA-۱۲۵b, Sirtuin, and Signal Transducer and Activator of Transcription ۳ with Biochemical Parameters and Risk Factors in Atherosclerosis Patients
- سال انتشار: 1402
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 12، شماره: 4
- کد COI اختصاصی: JR_RBMB-12-4_012
- زبان مقاله: انگلیسی
- تعداد مشاهده: 193
نویسندگان
Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Internal Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran & Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
چکیده
Background: Atherosclerosis (AS) is an inflammatory disease linked to vascular events, with dysregulation of microRNA (miR)-۱۲۵b, contributing to cardiovascular disease pathogenesis. Moreover, there is evidence of the involvement of signal transducer and activator of transcription ۳ (STAT۳) and sirtuin ۶ (SIRT۶) in AS. This study aimed to survey the expression levels of miR-۱۲۵b, STAT۳, and SIRT۶ in the peripheral blood mononuclear cells (PBMCs) of AS patients and controls, and to find their correlations with biochemical parameters and risk factors. Methods: This study included blood samples from ۴۵ controls and ۴۵ AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-۱۲۵b, STAT۳, and SIRT۶ were determined via quantitative Real Time-PCR. Results: The findings revealed a significant increase in miR-۱۲۵b levels in patients compared to controls (P = ۰.۰۱۷). However, alterations in STAT۳ and SIRT۶ expression were not significant (P> ۰.۰۵). There was no substantial relationship between miR-۱۲۵b and STAT۳ (P = ۰.۵۲۲) or SIRT۶ (P = ۰.۸۸). miR-۱۲۵b showed a significant relationship with atherogenic indexes and creatinine (P< ۰.۰۵), while the association of SIRT۶ with HDL and creatinine was significant (P< ۰.۰۵). STAT۳ exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P< ۰.۰۵). Conclusions: STAT۳ can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-۱۲۵b and SIRT۶ may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes. Keywords: Atherosclerosis, MicroRNA-۱۲۵b, STAT۳, SIRT۶, Leukocyte.کلیدواژه ها
Atherosclerosis, microRNA-۱۲۵b, Signal transducer and activator of transcription ۳, Sirtuin ۶, Leukocyteاطلاعات بیشتر در مورد COI
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