۵-Fluorouracil-Loaded PLGA Declined Expression of Pro-Inflammatory Genes IL-۹, IL-۱۷A, IL-۲۳ and IFN- γ in the HT-۲۹ Colon Cancer Cell Line
- سال انتشار: 1402
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 12، شماره: 4
- کد COI اختصاصی: JR_RBMB-12-4_015
- زبان مقاله: انگلیسی
- تعداد مشاهده: 70
نویسندگان
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Mohammad Ali Hosseinpour feizi
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Microbiology, Faculty of Medicine, Infectious Diseases Research Center, Gonabad University of Medical Science, Gonabad, Iran & Innovative Medical Research Center, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
چکیده
Background: Pro-inflammatory cytokines play critical roles in cancer pathobiology and have been considered potential targets for cancer management and therapy. Understanding the impact of cancer therapeutics such as ۵-fluorouracil (۵-FU) on their expression might shed light on development of novel combinational therapies. This study aimed to encapsulate ۵-FU into PLGA and evaluate their effects on the expression of pro-inflammatory genes IL-۹, IL-۱۷-A, IL-۲۳, and IFN-γ in the HT-۲۹ cells. Methods: PLGA-۵-FU NPs were constructed and characterized by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). The cytotoxicity was evaluated by MTT test and, the IC۵۰ was identified. HT-۲۹ cells were treated with different concentrations of the PLGA-۵-FU NPs for ۴۸ hours and, gene expression levels were analyzed by qRT-PCR. Results: DLS and AFM analysis revealed that the prepared PLGA-۵-FU NPs were negatively charged spherical-shaped particles with a mean size of ۲۱۵.۹ ± ۴۳.۳ nm. PLGA-۵-FU NPs impacted the viability of HT-۲۹ cells in a dose- and time-dependent manner. The qRT-PCR results revealed a dose-dependent decrease in the expression of IL-۹, IL-۱۷A, IL-۲۳ and IFN-γ genes, and their expressions were significantly different in both ۱۰ and ۲۰ µg/mL treated groups compared to the control. However, although the treatment of HT-۲۹ cells with ۲۰ µg/mL free ۵-FU resulted in decreased expression of the studied genes, the differences were not statistically significant compared to the control group. Conclusions: PLGA-۵-FU NPs significantly suppressed expression of the IL-۹, IL-۱۷A, IL-۲۳ and IFN-γ genes, and the encapsulation of ۵-FU into PLGA improved considerably impact of the ۵-FU on the HT-۲۹ cells.کلیدواژه ها
Cancer therapy, Colorectal cancer, Fluorouracil, Polylactic Acid-Polyglycolic Acid Copolymer (PLGA), Pro-inflammatory cytokine.اطلاعات بیشتر در مورد COI
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