Zahra Zeinali - Department of Microbiology, Faculty of Veterinary Medicine, Division of Immunology, Urmia University, Iran.
Leila Mahmoudzadeh - Department of Microbiology, Faculty of Veterinary Medicine, Division of Immunology, Urmia University, Iran.

Background and objective: Multiple studies on cancer showed that Mesenchymal Stem Cells (MSCs) inherently have different effects on various tumors. MSCs respond to multiple factors such as Caffeine, Estrogen, Morphine, curcumin, Metformin, and LPS due to the presence of different receptors. This study investigated the effects of caffeine, a popular drink, on the crosstalk between MSCs and ۴T۱ cells.Material and methods: Mesenchymal stem cells were isolated from mice's tibia and femur bones. These cells were cultured for ۱۴ days and subsequently treated with o as the control group, ۰.۱, ۰.۵, and ۱ mM doses of caffeine for ۲۴ h. Then, cells were cultured without fetal bovine serum (FBS) for ۲۴ h, and the conditioned medium(CM) was isolated. The ۴T۱ cells were cultured with CM for ۲۴ h, MTT reduction, and Neutral red uptake assay. The cytotoxicity and the rate of apoptosis and necrosis were detected by Acridin orang and PI staining. The presence of ۴T۱ cancer cells was detected using the flow cytometry method.Results and conclusion: The MSC treated with caffeine could have. The more cytotoxic effect on ۴T۱ cells compared to non-treated MSC was seen in a dose-dependent manner, such that in the group that received a ۰.۵ mM concentration of caffeine, the most significant impact was seen on cell line ۴.۱. This data showed that the CM of MSC, treated with caffeine, had a cytotoxic effect on ۴T۱ cells. Caffeine could suppress the growth rate of ۴T۱ cells. The ideal caffeine concentration is ۰.۵ mM.

Breast cancer stem Cell, Caffeine, Mesenchymal stem cell, ۴T۱cell line