Isolation, identification and assessment of bioremediation potential of oil-degrading bacteria from oil-polluted sites of south of Iran
- سال انتشار: 1391
- محل انتشار: همایش بین المللی بحران های زیست محیطی ایران و راهکارهای بهبود آن
- کد COI اختصاصی: ICECS01_044
- زبان مقاله: انگلیسی
- تعداد مشاهده: 1606
نویسندگان
Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Department of Soil Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
Department of Soil Biology, Institute of Soil & Water research, Karaj, Iran
چکیده
The pollution of the environment with oil is spreading throughout the world along with industrial progress. Scientists are looking for many years to find solution for removing contaminants from the soil and water environments. Today, the use of microorganism, for removing crude oil pollution from contaminated sites (bioremediation) is considered by scientists. The present study deals with isolation, identification and characterization of oil-degrading bacteria was isolated from oil-polluted sites of south of Iran (Bushehr province). Initially, a total of 90 isolates were screened from oil-polluted soils in Carbon Free Minimal Medium enriched by gas oil. The 19 isolates were selected based on high level of growth in solid medium. On the basis of morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis revealed that, the isolates were authentically identified as Stenotrophomonas maltophilia, Ralstonia sp., Vibrio sp., Sphingobacterium sp., Zymomonas sp.,Pseudomonas aeruginosa, Paracoccus sp., Pantoea sp.,Achromobacter xylosoxidans, Acinetobacter johnsonii, Serattia odorifera, Pseudomonas alcaligenes, Entrobacter cloacae, Pseudomonas stutzeri andChryseobacterium sp.. In order to determine the efficiency of these bacteria in hydrocarbon-degrading an inoculum of bacteria containing 108 cfu/ml was used in plate and liquid assays, which were performed in factorial experiment based on completely randomized design with 3 replications. The increasing of diameter of colony and the growth turbidity (OD600nm) as indicators for utilization of hydrocarbon was measured in solid and liquid assays, respectively. Results showed that in CFMM plate assay, highest diameter of colony in presence of toluene and phenanthrene were achieved by Chryseobacterium sp. and Sphingobacterium sp., while in liquid assay and in presence of three compounds Serattia odorifera and Entrobacter cloacae were efficient. Moreover, among three compounds highest degradability in bioremediation was related to gas oil, and it’s followed by phenanthrene and toluene, respectively.کلیدواژه ها
Bioremediation, Oil-degradation bacteria, Gas oil, Toluene, Phenanthrene, Minimalمقالات مرتبط جدید
- استفاده از مواد دوست دار محیطزیست بهعنوان جایگزین برای صنعت بستهبندی مواد غذایی
- مروری بر کاربرد چارچوبهای فلزی-آلی در جذب دیاکسید کربن
- کنترل بهینه یک مدل دینامیکی امکانات و تسهیلات گردشگری در جهت توسعه پایدار مبتنی بر تاثیرات عوامل محیطی
- بررسی موانع پیاده سازی استانداردهایISO با تاکید بر مدیریت سبز با استفاده از شبکههای عصبی عمیق کانولوشن
- استخراج رمزارز و چالش های آلودگی محیط زیست
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.