Extended-spectrum beta-lactamases detection and prevalence of blaTEM gene in clinical isolates of Klebsiella pneumoniae from hospitals in North of Iran
- سال انتشار: 1401
- محل انتشار: مجله علوم زیستی خاورمیانه، دوره: 20، شماره: 1
- کد COI اختصاصی: JR_CJES-20-1_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 171
نویسندگان
Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
Department of Biology, Sari Branch, Islamic Azad University, Sari, Iran
چکیده
Klebsiella pneumoniae is a member of the Enterobacteriaceae family which plays an important role in creating various infections in the community. These strains are resistant to multiple β-lactam antibiotics due to the production of β-lactamases enzyme (ESBL). The purpose of this study was to assess the phenotypic and genotypic characteristics of ESBL-producing K. pneumoniae strains from clinical specimens in three hospitals in Mazandaran, Iran. In this cross-sectional study, K. pneumoniae samples (N = ۱۰۰) were identified from different clinical specimens after standard biochemical and microbiological tests. Disc agar diffusion test was applied for antibiotic-resistant examinations. Phenotypic detection of ESBL-producing isolates was performed using mixed disk method. The presence of blaTEM gene was investigated in ESBL-producing isolates using PCR method. The ESBL test analysis was positive for ۴۰ isolates (۴۰%) of K. pneumoniae. The prevalence of blaTEM gene in ESBL-producing K. pneumoniae isolates was ۵۵%. Tetracycline, tobramycin, and ampicillin were the most active antibiotics against K. pneumoniae isolates, showing ۸۵%, ۸۱% and ۷۳% sensitivity, respectively. The highest antibiotic resistance in isolated K. pneumoniae was found for ceftriaxone (۴۸%) and cefotaxime (۴۶%) antibiotics. There was a significant correlation between ESBL production and K. pneumoniae isolates resistance to cefotaxime (p = ۰.۰۰۰), ceftazidime (p = ۰.۰۰۱), ciprofloxacin (p = ۰.۰۰۱), tobramycin (p = ۰.۰۴۴), and ceftriaxone (p = ۰.۰۰۰). The prevalence of ESBL-producing K. pneumoniae was high and increasing. The high prevalence of blaTEM gene in these isolates may be a reason for their pathogenesis and multiple-antibiotic resistance. Therefore, there is a need to develop the strategies to manage antibiotic resistance in these isolates.کلیدواژه ها
Beta-lactamase, BlaTEM gene, Klebsiella pneumoniae, PCR, Phenotypic and genotypic studiesاطلاعات بیشتر در مورد COI
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