Determination of optimum conditions for the production of the mother culture of the medicinal wild mushroom, Agaricus bellanniae isolated from hot Iraqi environment (Baghdad Governorate)

  • سال انتشار: 1401
  • محل انتشار: مجله علوم زیستی خاورمیانه، دوره: 20، شماره: 2
  • کد COI اختصاصی: JR_CJES-20-2_007
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 183
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نویسندگان

Batool Shakir Abed Almjalawi

General Directorate of Education in Karbala, Karbala, Iraq

Rukaibaa Ali Chechan

Department of Food Science, College of Agriculture Engineering Sciences, University of Baghdad, Baghdad, Iraq

Dina Suad Ali

Department of Food Technology, College of Agricultural Engineering Sciences, Salhaddin University, Salhaddin, Iraq

Uroba Abed Shama

Ministry of Agriculture, State Co. for Agricultural Supplies, Baghdad, Iraq

Ekhlas Mohammed Farhan

Ministry of Sciences and Technology, Baghdad, Iraq

چکیده

The agricultural wild mushroom, Agaricus bellanniae, is one of the new fungi that have been discovered for the first time during July and August ۲۰۱۶ in Baghdad Governorate, Iraq. Due to the lack of research studies on this fungus globally, particularly in Iraq. This study is considered as the first research about determining the optimal conditions for the production of the fungus mother culture. A. bellanniae was done by creating a local culture medium suitable for developing wild and edible agricultural fungi. The study is concerned with two aspects, which are: isolation, purification, and diagnosis of the wild mushroom strain under the genus Agaricus to obtain a pure isolate characteristic of the fungus A. bellanniae registered in the NCBI (MF۹۸۷۸۴۳). The second aspect was studying the optimal conditions for preparing the mother culture from the wild mushroom. The study also examined the effect of some environmental factors on the fungus mycelium growth rates, such as the medium concentration and size, pH, and temperature. The results showed that the medium prepared from tree leaves powder achieved the best growth rates for A. bellanniae, with a growth rate of ۰.۷۷ cm day-۱, followed by other media. Also, the best concentration for preparing these media was shown to be ۲۰ g L-۱. The best pH that can be adopted at the mycelium production stage for A. bellanniae, which achieved the highest growth rate, was at pH ۶. The best temperature for the development of the agricultural mushroom, regardless of the media type, was at ۳۰ ºC.

کلیدواژه ها

Agaricus bellanniae mushroom, Mk MF۹۸۷۸۴۳, Wild mushroom, Al-Salhiya district, Baghdad Governorate, Cordia myxa tree leaves

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