Experimental accelerating testicular tissue recovery post-methotrexate treatment in rats: A promising role of Sertoli cell-conditioned medium: An experimentl study

  • سال انتشار: 1403
  • محل انتشار: International Journal of Reproductive BioMedicine، دوره: 22، شماره: 4
  • کد COI اختصاصی: JR_IJRM-22-4_004
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 176
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نویسندگان

Aref Delkhosh

Department of Pathobiology, Faculty of Veterinary Medicine, Division of Pathology, Urmia University, Urmia, Iran.

Fatemeh Shabani

Midwifery Department, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran.

Masoud Delashoub

Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran. Department of Basic Sciences, Biotechnology Research Centre, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

چکیده

Background: Methotrexate (MET) is one of the most important chemotherapy agents used against various tumors and cancer diseases. One of the critical side effects of MET is inducing male infertility. Objective: The current study aimed to investigate Sertoli cell culture-conditioned medium (SCM) recovery effects on MET-induced conditions in rats. Materials and Methods: ۳۰ mature male Wistar rats were randomly divided into ۳ groups (n = ۱۰). In the first group, rats received normal saline intraperitoneally. In the second group, animals received MET (۱۰ mg/kg; intraperitoneally) once a week for ۲ wk. The rats in the third group (MET+SCM) received MET and a single injection of SCM for ۵۶ days post-MET administration. ۵۶ days later, serum, epididymis, and testicular tissue samples were collected, and the animals were euthanized. Sperm parameters, serum levels of luteinizing hormone, follicle-stimulating hormone, and testosterone were examined. The testicular tissues were stained using hematoxylin and eosin solution, and histopathological changes were analyzed. Results: The MET-induced condition resulted in significant pathological changes in the testis, decreased hormone levels, and downregulated sperm parameters. However, SCM injection improved hormonal levels, testicular changes, and sperm parameters. Conclusion: It can be concluded that a single intra-testicular SCM injection accelerates male reproductive system recovery post-MET treatment.

کلیدواژه ها

Sertoli cell, Conditioned culture media, Methotrexate, Testis, Histopathology, Rats., سلول سرتولی, محیط کشت شرایطی, متوترکسات, بیضه, هیستوپاتولوژی, موش صحرائی.

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