Functionalization of Carbon Nanotubes Loaded with Tamoxifen and Their Anticancer Potential against Human Breast Cancer Cells
- سال انتشار: 1402
- محل انتشار: مجله علوم پیشرفته زیست پزشکی، دوره: 14، شماره: 1
- کد COI اختصاصی: JR_JABS-14-1_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 60
نویسندگان
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده
Background & Objectives: Carbon nanotubes (CNTs) serve as molecular carriers for in vivo and in vitro delivery. Initial studies have suggested that nanotubes in drug delivery can enhance the therapeutic response to anti-cancer drugs. The present study intended to investigate the effect of CNTs carrying tamoxifen (TAM-CNTs) on the induction of apoptosis in the MDA-MB-۲۳۱ cell line. Materials & Methods: The cells were treated with various concentrations of TAM and TAM-CNTs. The IC۵۰ for these compounds was determined using a MTT assay. The cells were then treated with a lower concentration of IC۵۰. The BAX and BCL-۲ genes expression were evaluated by Real-Time PCR and Western blot. Flow cytometry was employed for evaluating apoptosis induction by TAM and TAM-CNTs. Results: The IC۵۰ value of TAM and TAM-CNTs in a ۴۸-hour period was ۶۶.۱۹ mg/mL and ۳۶.۵۹ mg/mL, respectively. The results demonstrated that BAX in the cells treated with TAM and TAM-CNTs was upregulated ۳.۶۴ and ۷.۸۸ times, respectively (P < ۰.۰۵). Conversely, BCL-۲ was downregulated ۳.۹۸ and ۵.۳۱ times (P < ۰.۰۵). Furthermore, Western blot experiments confirmed the expression of BAX and BCL-۲ proteins based on their gene expression. Flow cytometry results indicated that the viability of MDA-MB-۲۳۱ cells in the control group, TAM-treated, and TAM-CNTs-treated cells was ۹۵.۳%, ۶۴.۹%, and ۱۳.۷۵%, respectively. This suggests that TAM-CNTs significantly diminishes cell viability compared to TAM (P < ۰.۰۰۱). Conclusion: The findings revealed that TAM accompanied by CNTs exhibits a greater cytotoxic and apoptotic effect on MDA-MB-۲۳۱ cells.کلیدواژه ها
Tamoxifen, Carbon Nanotubes, Nano-delivery system, Apoptosisاطلاعات بیشتر در مورد COI
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