Ricardo Hernández Pérez - National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. ۲۷, Col. Center, Zacatepec, Morelos ۶۲۷۸۰, Mexico
René Salgado Delgado - National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. ۲۷, Col. Center, Zacatepec, Morelos ۶۲۷۸۰, Mexico
Alfredo Olarte Paredes - National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. ۲۷, Col. Center, Zacatepec, Morelos ۶۲۷۸۰, Mexico
Areli Marlen Salgado Delgado - National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. ۲۷, Col. Center, Zacatepec, Morelos ۶۲۷۸۰, Mexico
Beatriz Flores Abarca - National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. ۲۷, Col. Center, Zacatepec, Morelos ۶۲۷۸۰, Mexico
Oswaldo E. Silva Astudillo - National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. ۲۷, Col. Center, Zacatepec, Morelos ۶۲۷۸۰, Mexico

Rice husk (Oryza sativa L.) is considered an agroindustrial waste that requires alternative treatment to prevent it from becoming an environmental contaminant. Some recent reports have been able to produce Nanocellulose (CNCs) with this biomass, although with low yields, after using a complex of endo and exoenzymes. Work aimed to evaluate the previous effect of natural proteolytic enzymes (Bromelain/Papain) to degrade the fibrous structure of rice husk before obtaining Cellulose and for whitening, an alternative with hydrogen peroxide. In combination with subsequent enzymatic hydrolysis the new complex formed by four reagents: D-(+)-Cellobiose, endo-۱,۴-ꞵ-D glucanase, ꞵ-glucosidase, and Cellobiohydrolase, for the synthesis of Nanocrystals (CNCs). Satisfactory results indicated that it was possible to soften and modify the plant tissue structure, which allowed a final cellulose yield of ۵۶.۳۵%. The hydrolysis with the enzyme complex applied to Cellulose, achieved the obtaining of CNCs between ۲۰۰-۵۹۵ nm, after using the four enzymes in complex, with better results when used in a single phase, achieving a final yield of ۱.۸ -۴.۲% nanocellulose with process optimization. google is broken

Oryza sativa L, agroindustrial waste, Enzymes, bromelain, papain