Bioinformatic-based Study to Investigate the Structure and Function of Pro-inflammatory Cytokines TNFα and IL-۶ Involved in the Pathogenesis of COVID-۱۹

Background & Objective: Besides the clinical and laboratory research on the COVID-۱۹ virus, the bioinformatics study in the field of genetics of immunity to COVID-۱۹ is of particular importance. In this account, studies show that in patients with COVID-۱۹, the level of tumor necrosis alpha (TNFα) and interleukin-۶ (IL-۶) is high and in severe cases of COVID-۱۹, the production of IL-۶, TNF-α, and other cytokines increases profoundly. On the other hand, investigating the molecular structure and receptors of IL-۶ and TNFα and the structural analysis of the receptor proteins may potentially help to develop new therapeutic plans for COVID-۱۹ infection.Methods: To identify genes with significant and different expressions in patients with COVID-۱۹ in a microarray data set containing transcriptional profiles from GEO as a functional genomic database the GEO query package version ۲.۶۴.۲ in a programming language R version ۴.۲.۱ was downloaded. In this way, functional enrichment analysis for DEGs, WikiPathways, REGO, gene ontology, and STRING database was also investigated and employed.Results: The structure and function of pro-inflammatory cytokines TNFα and IL-۶ involved in the pathogenesis of COVID-۱۹ were investigated, and in general, after performing various analyses in this study and extracting A series of genes with different expressions from the KEGG database, the final ۵ DEGs include CXCL۱۴, CXCL۶, CCL۸, CXCR۱, TNFRSF۱۰, and the relationship and expression effects of them were observed in different pathways.Conclusion: IL-۶ and TNFα were involved in immunological processes that had a direct and indirect relationship with the activation of cytokines, including IL۶ and TNF-a, and cytokine storm, and this indicates their role in the formation of problems and complications, including ARDS, in COVID-۱۹ patients. Of course, determining the effectiveness of each of these genes requires more specialized and clinical studies.

کلیدواژه ها:

Bioinformatics ، COVID-۱۹ ، Cytokine ، IL-۶ ، TNF-a