Recombinant Production of a Chimeric Protein Containing StxB, Eae, Cfab, and LTB Proteins in Hairy Roots of Nicotiana tabaccum
- سال انتشار: 1403
- محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 11، شماره: 1
- کد COI اختصاصی: JR_JABR-11-1_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 189
نویسندگان
Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
چکیده
Introduction: The technology of recombinant protein production has revolutionized biological, pharmaceutical, and medical researches. Plants are appropriate systems for the production of recombinant proteins. In the present study, a chimeric gene encoding a chimeric protein containing StxB, Eae, CfaB, and LTB as a candidate vaccine against enterotoxigenic and enterohemorrhagic E. coli, was transferred to the hairy root of the tobacco plant and its expression was analyzed.Materials and Methods: For this aim, the plant-based codon-optimized gene, which was synthetically cloned in pBI۱۲۱ binary vector, was transferred into Agrobacterium rhizogenes competent cells. A. rhizogenes cells containing pBI۱۲۱+secl were transferred into sterile Nicotiana tabaccum plants by direct and indirect methods. Finally, the expression of recombinant protein was analyzed by the ELISA method.Results: The transformation of A. rhizogenes cells and Tobacco plants was confirmed by PCR amplification of a fragment of the chimeric gene with specific primers. SDS-PAGE and ELISA analysis showed the successful expression of the chimeric recombinant protein.Conclusions: In conclusion, it was shown that the hairy roots of tobacco plant is a proper expression system for the recombinant production of SECL protein. The protein can be used for mice's immunization against enterotoxigenic and enterohemorrhagic E. coli strains.کلیدواژه ها
Protein expression, Hairy Roots, Nicotiana tabaccum, Vaccine candidateاطلاعات بیشتر در مورد COI
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