Introduction of yeast species from tomato plant flora

  • سال انتشار: 1402
  • محل انتشار: هفتمین کنگره سالانه بین المللی توسعه کشاورزی، منابع طبیعی، محیط زیست و گردشگری ایران
  • کد COI اختصاصی: ICSDA07_003
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 109
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نویسندگان

Turaj Nader

Student, Sari University of Natural Resources and Agricultural Sciences

Mohammad Ali Tajik Ghanbari

Associate Professor, Sari University of Natural Resources and Agricultural Sciences

Lachin Mokhtar Nejad

Assistant Professor, Center for Research and Education of Agriculture and Natural Resources of West Azerbaijan

Syamak Hanifeh

Consultant, West Azerbaijan Agriculture and Natural Resources Research and Education Center

چکیده

Yeasts as unicellular organisms have been of special importance in the biocontrol of agricultural productssince long ago. These organisms are able to settle on an environment for a long time by maintaining theirdeterrent power. So far, different species of yeasts have been identified. In this study, in order to introducetomato flora yeasts, sampling was done from the fruit, leaves and roots of this plant from severalgreenhouses. All isolates were isolated and purified in DRBC and PDA media and were grouped based onmacroscopic characteristics (color and colony shape), and ۱۷ isolates were identified. All isolates wereevaluated for the presence of secreted enzymes. ۵ isolates of protease production, ۶ isolates of pectinaseand starch production and esterase production were not observed in any of the isolates. In the molecularand phenotypic analysis of the sequence of D۱/D۲ region of ۲۸S rRNA gene, four isolates were introducedwith PCR۳R and V۹ primers. Introduced isolates include: Kabatiella microsticta, Blastobotrys sp.,Cytobasidium terricola, Naganishia vishniacii

کلیدواژه ها

yeast, identification, tomato flora, enzyme activity

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