The in Vitro Effect of Berberine Sulfate and Berberine Chloride on the Growth and Aflatoxin Production by Aspergillus flavus and Aspergillus parasiticus
- سال انتشار: 1403
- محل انتشار: فصلنامه طب دامی ایران، دوره: 18، شماره: 2
- کد COI اختصاصی: JR_IJVM-18-2_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 34
نویسندگان
Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Division of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
چکیده
Background: Aflatoxins are harmful mycotoxins that can contaminate animal and human food products. To prevent toxigenic fungi growth and aflatoxin production, researchers have long investigated plant compounds as potential inhibitory agents.Objectives: This study aimed to evaluate the in vitro effect of berberine sulfate and berberine chloride on the growth and aflatoxin production of Aspergillus flavus and Aspergillus parasiticus.Methods: The antifungal activity of berberine salts was determined according to the Clinical and Laboratory Standards Institute (CLSI) document M۳۸-A۳. The aflatoxin levels were measured using high-performance liquid chromatography (HPLC) method.Results: The berberine sulfate and berberine chloride showed inhibitory effects against both Aspergillus species, with minimum inhibitory concentration (MIC) ranging from ۱۲۵ to ۵۰۰ µg/mL. Berberine sulfate at ۲۰۰۰ μg/mL and berberine chloride at ۱۰۰۰ μg/mL completely inhibited the mycelial growth of A. flavus, while berberine chloride at ۱۰۰۰ μg/mL also completely inhibited the mycelial growth of A. parasiticus. Berberine sulfate at ۲۰۰۰ µg/mL reduced the mycelial growth of A. parasiticus by ۹۶.۷%. Conclusion: Berberine salts significantly decreased the total aflatoxin production of Aspergillus species at MIC/۲ and MIC/۴ concentrations (P˂۰.۰۵). The results suggest that berberine salts could be used as potential antifungal and anti-aflatoxigenic agents against toxigenic Aspergillus isolates.کلیدواژه ها
Aflatoxins, Aspergillus flavus, Aspergillus parasiticus, Berberine, Mycelial growthاطلاعات بیشتر در مورد COI
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